Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits.

M. Musalmah, J. Normah, W. B. Mohamad, O. N. Salwah, H. A. Fatah, N. A. Zahari

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effect of HbE, a hemoglobin variant, on the determination of HbA1/HbA1c using 4 commercial kits based on cation-exchange resin, cation-exchange column chromatography and specific antibody techniques was studied. Fifty-eight normal and 63 HbE heterozygous subjects were tested for HbA1 and HbA1c using 4 commercial kits i.e. Eagles Diagnostics, Boehringer Mannehim (BM), Diastat and Ames DCA 2000. Analyses of the samples by the 4 kits were done within one week and samples were stored at 4 degrees C before analysis. The results showed that HbE affects the determination of glycosylated hemoglobin using cation-exchange based and not kits based on specific antibody techniques.

Original languageEnglish
Pages (from-to)352-356
Number of pages5
JournalMedical Journal of Malaysia
Volume55
Issue number3
Publication statusPublished - Sep 2000
Externally publishedYes

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Glycosylated Hemoglobin A
Cations
Cation Exchange Resins
Eagles
Antibodies
Chromatography
Hemoglobins
glycosylated hemoglobin E

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Musalmah, M., Normah, J., Mohamad, W. B., Salwah, O. N., Fatah, H. A., & Zahari, N. A. (2000). Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits. Medical Journal of Malaysia, 55(3), 352-356.

Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits. / Musalmah, M.; Normah, J.; Mohamad, W. B.; Salwah, O. N.; Fatah, H. A.; Zahari, N. A.

In: Medical Journal of Malaysia, Vol. 55, No. 3, 09.2000, p. 352-356.

Research output: Contribution to journalArticle

Musalmah, M, Normah, J, Mohamad, WB, Salwah, ON, Fatah, HA & Zahari, NA 2000, 'Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits.', Medical Journal of Malaysia, vol. 55, no. 3, pp. 352-356.
Musalmah M, Normah J, Mohamad WB, Salwah ON, Fatah HA, Zahari NA. Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits. Medical Journal of Malaysia. 2000 Sep;55(3):352-356.
Musalmah, M. ; Normah, J. ; Mohamad, W. B. ; Salwah, O. N. ; Fatah, H. A. ; Zahari, N. A. / Effect of hemoglobin E on glycosylated hemoglobin determinations using different commercial kits. In: Medical Journal of Malaysia. 2000 ; Vol. 55, No. 3. pp. 352-356.
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