Effect of Growth Factor Supplementation on the Hair Cell Specific Markers of Cells Harvested from Basilar Membrane

Muhd A zhan Ubaidah, Chua Kien Hui, Mazita Ami, Azida Zainal, Aminuddin Saim, Lokman Saim, Lokanathan Yogeswaran, Ruszymah Idrus

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: Loss of auditory hair cells is a major cause of deafness. The presence of auditory progenitor cells in the inner ear raises the hope for mammalian inner ear cell regeneration. In this study, we aimed to investigate the effect of growth factor supplementations, namely a combination of epidermal growth factor (EGF), insulin-like growth factor (IGF), and beta (β)-fibroblast growth factor (βFGF), on the expression of hair cell-specific markers by cells harvested from the cochlear membrane. This would provide an insight into the capability of these cells to differentiate into hair cells.

MATERIALS AND METHODS: EGF, IGF, and βFGF were supplemented into the culture medium. The cells were evaluated by morphology, growth kinetic, gene expression, and protein expression.

RESULTS: The cultured cells of mouse basilar membrane were spindle shaped. Growth factors-enriched medium promotes a significantly higher proliferative activity than the basic culture medium but did not alter the cell morphology. Growth factors-enriched medium did not show any significant differences in the protein expression of the hair cell-specific markers myosin VIIa and calretinin and the stem-cell marker nestin. Gene expression analysis showed that the expression of the hair cell-specific genes myosin VIIa and calretinin as well as the stem cell genes nestin, Rex1, and Sox2 was reduced after the cells were passaged in the growth factor-supplemented medium. Cells in the basic medium expressed a significantly higher level of hair cell-specific genes at certain passages.

CONCLUSION: Growth factor supplementation could not maintain the expression of hair cell-specific markers by cells obtained from the cochlear membrane.

Original languageEnglish
Pages (from-to)23-29
Number of pages7
JournalThe journal of international advanced otology
Volume11
Issue number1
DOIs
Publication statusPublished - 1 Apr 2015

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Basilar Membrane
Intercellular Signaling Peptides and Proteins
Calbindin 2
Nestin
Stem Cells
Fibroblast Growth Factors
Cochlea
Somatomedins
Inner Ear
Epidermal Growth Factor
Culture Media
Auditory Hair Cells
Genes
Gene Expression
Membranes
Deafness

ASJC Scopus subject areas

  • Medicine(all)

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Effect of Growth Factor Supplementation on the Hair Cell Specific Markers of Cells Harvested from Basilar Membrane. / Ubaidah, Muhd A zhan; Kien Hui, Chua; Ami, Mazita; Zainal, Azida; Saim, Aminuddin; Saim, Lokman; Yogeswaran, Lokanathan; Idrus, Ruszymah.

In: The journal of international advanced otology, Vol. 11, No. 1, 01.04.2015, p. 23-29.

Research output: Contribution to journalArticle

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