Effect of gonadectomy and sex hormones replacement on glutathione related enzymes in rats

H. Zarida, W. Z. Wan Ngah, B. A K Khalid

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    The effects of gonadectomy and sex hormone replacement (either estradiol (E) or progesterone (P) or both (EP) in females, and testosterone (T) in males) on plasma and hepatic gamma-glutamyl transpeptidase (PGGT and MGGT respectively), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GRx) were investigated. 48 male and 72 female rats (120-150 g each) were used. The male rats were divided into 4 groups: control, sham operated, castrated and castrated with testosterone replacement at a dose of 4 mg kg-1 body weight. The female rats were divided into 6 groups: control, sham operated and 4 groups which were gonadectomized, of which three groups received hormone replacements of estradiol (0.2 mg kg-1 body weight), progesterone 4 mg kg-1 body weight) and both estradiol and progesterone together. The animals were sacrificed after two and five months. Higher levels of GPx and GRx activities (p < 0.01) were found in female rats whereas GST were higher in male rats. Although no differences were observed in PGGT activities between male and female rats, MGGT activities were higher in female rats at five months. Ovariectomy increased PGGT, GST and GRx activities and replacement with either E or P lowered the activities. Replacement with EP together brought the levels of plasma GGT, GST and GRx to that of intact females. Neither gonadectomy nor sex hormone replacement influenced hepatic GGT and GPx activities. Castration increased the hepatic GGT and GPx activities of male rats, but reduced the GST levels. Testosterone replacement brought the hepatic GGT and GPx activities to that of intact males. Castration and or testosterone replacement did not influence the levels of plasma GGT and GRx. The results suggest that GGT and GSH-related enzymes are subjected to modulation by male and female sex hormones.

    Original languageEnglish
    Pages (from-to)223-230
    Number of pages8
    JournalAsia Pacific Journal of Pharmacology
    Volume8
    Issue number4
    Publication statusPublished - 1993

    Fingerprint

    Gonadal Steroid Hormones
    Glutathione
    Glutathione Peroxidase
    Glutathione Transferase
    Enzymes
    Testosterone
    Progesterone
    Estradiol
    Liver
    Castration
    Body Weight
    Control Groups
    gamma-Glutamyltransferase
    Glutathione Reductase
    Ovariectomy
    Hormones

    Keywords

    • castration
    • estrogen
    • gamma-glutam yltranspeptidase
    • glutathione
    • glutathione peroxidase
    • glutathione reductase
    • glutathione S-transferase
    • ovariectomy
    • progesterone
    • testosterone

    ASJC Scopus subject areas

    • Pharmacology

    Cite this

    Effect of gonadectomy and sex hormones replacement on glutathione related enzymes in rats. / Zarida, H.; Wan Ngah, W. Z.; Khalid, B. A K.

    In: Asia Pacific Journal of Pharmacology, Vol. 8, No. 4, 1993, p. 223-230.

    Research output: Contribution to journalArticle

    Zarida, H. ; Wan Ngah, W. Z. ; Khalid, B. A K. / Effect of gonadectomy and sex hormones replacement on glutathione related enzymes in rats. In: Asia Pacific Journal of Pharmacology. 1993 ; Vol. 8, No. 4. pp. 223-230.
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