Effect of gamma irradiation on the physical stability of DPPC liposomes

Liyana Mohd Ali Napia, Irman Abdul Rahman, Mohd Yusof Hamzah, Faizal K.P. Kunchi Mohamed, Hur Munawar Kabir Mohd, Intan Syakeela Ahmad Bastamam, Shamellia Sharin, Norsyahidah Mohd Hidzir, Shahidan Radiman

Research output: Contribution to journalArticle

Abstract

Unilamellar liposomes composed of dipalmitoylphosphatidylcholine (DPPC) were prepared by the reverse-phase evaporation method and extrusion through a polycarbonate membrane filter. Liposomes at 0.7 mg/mL lipid concentration in deionized water were exposed to gamma irradiation at a dose in the range 0.5 to 25 kGy. Gamma irradiation of liposomes resulted in the degradation of DPPC lipids into free fatty acids, lysophosphatidylcholine and 1,2-palmitoyl-phosphatidic acid (DPPA). The effect of gamma irradiation towards the physical stability of liposomes was investigated by means of dynamic light scattering (DLS), transmission electron microscopy (TEM) and zeta potential analysis. From the DLS analysis, no significant changes were observed in the hydrodynamic size of liposomes. TEM images indicate that the liposomes surface became smoother and rounder as higher irradiation doses were applied. Zeta potential analysis showed that gamma irradiation of DPPC liposomes at radiation doses as low as 0.5 kGy resulted in a drastic rise in the magnitude of the zeta potential. The results also demonstrate that gamma irradiation of liposomes suspension enhanced the overall stability of liposomes. Hence, it can be concluded that gamma irradiation on DPPC liposomes may potentially produce liposomes with higher stability.

Original languageEnglish
Pages (from-to)1235-1240
Number of pages6
JournalSains Malaysiana
Volume47
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018

Fingerprint

irradiation
dosage
lipids
light scattering
transmission electron microscopy
polycarbonates
fatty acids
hydrodynamics
evaporation
degradation
membranes
filters
acids
radiation
water

Keywords

  • Dipalmitoylphosphatidylcholine (DPPC)
  • Gamma irradiation
  • Physical stability
  • Zeta potential

ASJC Scopus subject areas

  • General

Cite this

Napia, L. M. A., Abdul Rahman, I., Hamzah, M. Y., K.P. Kunchi Mohamed, F., Mohd, H. M. K., Bastamam, I. S. A., ... Radiman, S. (2018). Effect of gamma irradiation on the physical stability of DPPC liposomes. Sains Malaysiana, 47(6), 1235-1240. https://doi.org/10.17576/jsm-2018-4706-19

Effect of gamma irradiation on the physical stability of DPPC liposomes. / Napia, Liyana Mohd Ali; Abdul Rahman, Irman; Hamzah, Mohd Yusof; K.P. Kunchi Mohamed, Faizal; Mohd, Hur Munawar Kabir; Bastamam, Intan Syakeela Ahmad; Sharin, Shamellia; Mohd Hidzir, Norsyahidah; Radiman, Shahidan.

In: Sains Malaysiana, Vol. 47, No. 6, 01.06.2018, p. 1235-1240.

Research output: Contribution to journalArticle

Napia, Liyana Mohd Ali ; Abdul Rahman, Irman ; Hamzah, Mohd Yusof ; K.P. Kunchi Mohamed, Faizal ; Mohd, Hur Munawar Kabir ; Bastamam, Intan Syakeela Ahmad ; Sharin, Shamellia ; Mohd Hidzir, Norsyahidah ; Radiman, Shahidan. / Effect of gamma irradiation on the physical stability of DPPC liposomes. In: Sains Malaysiana. 2018 ; Vol. 47, No. 6. pp. 1235-1240.
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