Effect of DNA type on response of DNA biosensor for carcinogens

Nor Diyana Bt Md Sani, Yook Heng Lee, Salmijah Surif, Azwani Mat Lazimb

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Carcinogens are cancer causing chemicals that can bind to DNA and cause damage to the DNA. These chemicals are available everywhere including in water, air, soil and food. Therefore, a sensor that can detect the presence of these chemicals will be a very useful tool. Since carcinogens bind to DNA, DNA can be used as the biological element in a biosensor. This study has utilized different types of DNA in a biosensor for carcinogen detection. The DNAs include double stranded calf thymus DNA, single stranded calf thymus DNA and guanine rich single stranded DNA. The modified SPE was exposed to a carcinogen followed by interaction with methylene blue which acts as the electroactive indicator. The SPE was then analysed using differential pulse voltammetry (DPV). Optimization studies were conducted for MB concentration and accumulation time, DNA concentration, as well as effect of buffer concentration, buffer pH and ionic strength. The performance of the biosensor was tested on a group 1 carcinogen, formaldehyde. The results indicated that the usage of guanine rich single stranded DNA also gives higher response as carcinogens prefer to bind with guanine compared to other bases.

Original languageEnglish
Title of host publicationAIP Conference Proceedings
Pages636-640
Number of pages5
Volume1571
DOIs
Publication statusPublished - 2013
Event2013 UKM Faculty of Science and Technology Post-Graduate Colloquium - Selangor
Duration: 3 Jul 20134 Jul 2013

Other

Other2013 UKM Faculty of Science and Technology Post-Graduate Colloquium
CitySelangor
Period3/7/134/7/13

Fingerprint

carcinogens
bioinstrumentation
deoxyribonucleic acid
guanines
calves
buffers
methylene blue
formaldehyde
food
soils

Keywords

  • Carcinogens
  • DNA
  • DNA biosensor

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Sani, N. D. B. M., Lee, Y. H., Surif, S., & Lazimb, A. M. (2013). Effect of DNA type on response of DNA biosensor for carcinogens. In AIP Conference Proceedings (Vol. 1571, pp. 636-640) https://doi.org/10.1063/1.4858726

Effect of DNA type on response of DNA biosensor for carcinogens. / Sani, Nor Diyana Bt Md; Lee, Yook Heng; Surif, Salmijah; Lazimb, Azwani Mat.

AIP Conference Proceedings. Vol. 1571 2013. p. 636-640.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sani, NDBM, Lee, YH, Surif, S & Lazimb, AM 2013, Effect of DNA type on response of DNA biosensor for carcinogens. in AIP Conference Proceedings. vol. 1571, pp. 636-640, 2013 UKM Faculty of Science and Technology Post-Graduate Colloquium, Selangor, 3/7/13. https://doi.org/10.1063/1.4858726
Sani NDBM, Lee YH, Surif S, Lazimb AM. Effect of DNA type on response of DNA biosensor for carcinogens. In AIP Conference Proceedings. Vol. 1571. 2013. p. 636-640 https://doi.org/10.1063/1.4858726
Sani, Nor Diyana Bt Md ; Lee, Yook Heng ; Surif, Salmijah ; Lazimb, Azwani Mat. / Effect of DNA type on response of DNA biosensor for carcinogens. AIP Conference Proceedings. Vol. 1571 2013. pp. 636-640
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