Effect of different hydrolysis time and enzymes on chemical properties, antioxidant and antihyperglycemic activities of edible bird nest hydrolysate

Alia Syafieqah Zulkifli, Abd. Salam Babji, Seng Joe Lim, Arnida Hani Teh, Norlida Mat Daud @ Daud, Hafeedza Abdul Rahman

Research output: Contribution to journalArticle

Abstract

Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of swiftlet species commonly found in the Southeast Asian region, including Malaysia. It is consumed traditionally by the Chinese as food delicacy and also considered as an important ingredient in traditional Chinese medicine for its beneficial health effects. The aim of this study was to evaluate the effect of enzymatic hydrolysis using alcalase, papain and papaya juice hydrolyze at 0.5 to 3 hr on the degree of hydrolysis (DH), protein solubility, concentration of reducing sugar, antioxidant and anti-hyperglycemic activity. In general, an increase in hydrolysis time increases the DH and soluble protein digestion rate. However, the concentration of reducing sugar was not affected by hydrolysis time. The solubility of proteins was highest for alcalase and papain at 3 h, while papaya juice at 2 h. Papain showed the highest antioxidant activity in 1 and 2 h of hydrolysis time whereas at 3 h, results for both papain and alcalase were higher compared to papaya juice. Result of anti-hyperglycemic activity showed that only EBN hydrolyzed using papaya juice has positive activity. Based on this study, enzymatic hydrolysis had improved the functional properties of EBN and results showed the potential of EBN to be developed as natural antioxidants and anti-hyperglycemic agents.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalMalaysian Applied Biology
Volume48
Issue number2
Publication statusPublished - 1 May 2019

Fingerprint

hypoglycemic agents
hydrolysates
Hypoglycemic Agents
papain
Birds
papayas
physicochemical properties
Hydrolysis
Antioxidants
Carica
hydrolysis
subtilisin
juices
Papain
antioxidants
Subtilisins
Enzymes
enzymes
glycemic effect
protein solubility

Keywords

  • Anti-hyperglycemic
  • Antioxidant
  • Degree of hydrolysis
  • Edible bird nest
  • Hydrolysate

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

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title = "Effect of different hydrolysis time and enzymes on chemical properties, antioxidant and antihyperglycemic activities of edible bird nest hydrolysate",
abstract = "Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of swiftlet species commonly found in the Southeast Asian region, including Malaysia. It is consumed traditionally by the Chinese as food delicacy and also considered as an important ingredient in traditional Chinese medicine for its beneficial health effects. The aim of this study was to evaluate the effect of enzymatic hydrolysis using alcalase, papain and papaya juice hydrolyze at 0.5 to 3 hr on the degree of hydrolysis (DH), protein solubility, concentration of reducing sugar, antioxidant and anti-hyperglycemic activity. In general, an increase in hydrolysis time increases the DH and soluble protein digestion rate. However, the concentration of reducing sugar was not affected by hydrolysis time. The solubility of proteins was highest for alcalase and papain at 3 h, while papaya juice at 2 h. Papain showed the highest antioxidant activity in 1 and 2 h of hydrolysis time whereas at 3 h, results for both papain and alcalase were higher compared to papaya juice. Result of anti-hyperglycemic activity showed that only EBN hydrolyzed using papaya juice has positive activity. Based on this study, enzymatic hydrolysis had improved the functional properties of EBN and results showed the potential of EBN to be developed as natural antioxidants and anti-hyperglycemic agents.",
keywords = "Anti-hyperglycemic, Antioxidant, Degree of hydrolysis, Edible bird nest, Hydrolysate",
author = "Zulkifli, {Alia Syafieqah} and Babji, {Abd. Salam} and Lim, {Seng Joe} and Teh, {Arnida Hani} and {Mat Daud @ Daud}, Norlida and Rahman, {Hafeedza Abdul}",
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T1 - Effect of different hydrolysis time and enzymes on chemical properties, antioxidant and antihyperglycemic activities of edible bird nest hydrolysate

AU - Zulkifli, Alia Syafieqah

AU - Babji, Abd. Salam

AU - Lim, Seng Joe

AU - Teh, Arnida Hani

AU - Mat Daud @ Daud, Norlida

AU - Rahman, Hafeedza Abdul

PY - 2019/5/1

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N2 - Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of swiftlet species commonly found in the Southeast Asian region, including Malaysia. It is consumed traditionally by the Chinese as food delicacy and also considered as an important ingredient in traditional Chinese medicine for its beneficial health effects. The aim of this study was to evaluate the effect of enzymatic hydrolysis using alcalase, papain and papaya juice hydrolyze at 0.5 to 3 hr on the degree of hydrolysis (DH), protein solubility, concentration of reducing sugar, antioxidant and anti-hyperglycemic activity. In general, an increase in hydrolysis time increases the DH and soluble protein digestion rate. However, the concentration of reducing sugar was not affected by hydrolysis time. The solubility of proteins was highest for alcalase and papain at 3 h, while papaya juice at 2 h. Papain showed the highest antioxidant activity in 1 and 2 h of hydrolysis time whereas at 3 h, results for both papain and alcalase were higher compared to papaya juice. Result of anti-hyperglycemic activity showed that only EBN hydrolyzed using papaya juice has positive activity. Based on this study, enzymatic hydrolysis had improved the functional properties of EBN and results showed the potential of EBN to be developed as natural antioxidants and anti-hyperglycemic agents.

AB - Edible bird nest (EBN) is a dried glutinous secretion from the salivary glands of swiftlet species commonly found in the Southeast Asian region, including Malaysia. It is consumed traditionally by the Chinese as food delicacy and also considered as an important ingredient in traditional Chinese medicine for its beneficial health effects. The aim of this study was to evaluate the effect of enzymatic hydrolysis using alcalase, papain and papaya juice hydrolyze at 0.5 to 3 hr on the degree of hydrolysis (DH), protein solubility, concentration of reducing sugar, antioxidant and anti-hyperglycemic activity. In general, an increase in hydrolysis time increases the DH and soluble protein digestion rate. However, the concentration of reducing sugar was not affected by hydrolysis time. The solubility of proteins was highest for alcalase and papain at 3 h, while papaya juice at 2 h. Papain showed the highest antioxidant activity in 1 and 2 h of hydrolysis time whereas at 3 h, results for both papain and alcalase were higher compared to papaya juice. Result of anti-hyperglycemic activity showed that only EBN hydrolyzed using papaya juice has positive activity. Based on this study, enzymatic hydrolysis had improved the functional properties of EBN and results showed the potential of EBN to be developed as natural antioxidants and anti-hyperglycemic agents.

KW - Anti-hyperglycemic

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