Effect of different drying methods and solvents on phenolic content, antioxidant and anti-hyperglycemic activities of Melastoma malabathricum leaves extract

Erna Nazirah Zahwal, Chen Pei Fang, Nurul Najiha Ain Ibrahim, Noorul Syuhada Mohd Razali, Hafeedza Abdul Rahman

Research output: Contribution to journalArticle

Abstract

Medicinal plants have been used as anti-diabetic agents due to their excellent antioxidant properties and polyphenolic compounds. This study was carried out to determine the effects of different solvents (ethanol:water ratio of 100:0. 50:50 and 0:100) and drying methods (freeze dry (FD), oven dry (OD) and air dry (AD)) on phenolic content, antioxidant and anti-hyperglycemic activities of Melastoma malabathricum. The antioxidant activities were determined by DPPH radical scavenging test, ferric reducing antioxidant power (FRAP) test, total phenolic content (TPC) while the anti-hyperglycemic activity were measured by inhibition of α-glucosidase. A trend can be observed whereby M. malabathricum extracted with 100% ethanol showed significantly higher phenolic content and greater antioxidant capacity compared to 50:50 and 100% water extract in all drying techniques which indicates as the best solvent for extracting compound responsible for the activities measured. FD M. malabathricum extracted with 100% ethanol showed lowest IC50 (DPPH) value (21.30 µg/mL), highest antioxidant activity in FRAP (3.85 mg TEAC/g extract) and α-glucosidase inhibition (95.74%). Highest phenolic content (382.19 mg GAE/g extract) also was shown for OD M. malabathricum extracted with 100% ethanol. Positive correlation between TPC with antioxidant and anti-hyperglycemic activities showed that M. malabathricum have potential as a source of natural antioxidant and anti-hyperglycemic agent with the presence of phenolic compounds.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalMalaysian Applied Biology
Volume47
Issue number5
Publication statusPublished - 1 Nov 2018

Fingerprint

Melastoma malabathricum
glycemic effect
leaf extracts
Antioxidants
drying
antioxidants
ethanol
hypoglycemic agents
Ethanol
glucosidases
Glucosidases
ovens
extracts
antioxidant activity
Freeze Drying
Water
medicinal plants
inhibitory concentration 50
phenolic compounds
polyphenols

Keywords

  • Anti-hyperglycemic
  • Antioxidant
  • Drying method
  • Melastoma malabathricum
  • Phenolic content

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Effect of different drying methods and solvents on phenolic content, antioxidant and anti-hyperglycemic activities of Melastoma malabathricum leaves extract. / Zahwal, Erna Nazirah; Fang, Chen Pei; Ibrahim, Nurul Najiha Ain; Mohd Razali, Noorul Syuhada; Rahman, Hafeedza Abdul.

In: Malaysian Applied Biology, Vol. 47, No. 5, 01.11.2018, p. 47-53.

Research output: Contribution to journalArticle

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