Effect of curcumin on aortic changes in ovariectomized rats fed with repeatedly heated soy oil

A preliminary electron microscopic study

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5 Citations (Scopus)

Abstract

Background and aims: Consumption of repeatedly heated soy oil has been linked with incidence of atherosclerosis particularly in oestrogen deficient states. In the present study, effect of curcumin extract on the prevention of atherosclerosis was evaluated. Materials and Methods: Forty eight female Spraque-Dawley rats (weighing 200-250 gm) were divided into eight groups. All groups were fed with 2% cholesterol diet. The sham control groups consisted of vitamin E free-RBD Olein (IV62) that acted as vehicle, and curcumin treated groups without undergoing ovariectomy. The other six groups were subjected to ovariectomy and later treated with vehicle-only, curcumin-only, once heated soy oil (1HSO) with vehicle, 1HSO with curcumin, five times heated soy oil (5HSO) with vehicle and 5HSO with curcumin. Curcumin was administered orally at a dose of 50 mg/kg which was commenced two weeks following ovariectomy. Following four months, the rats were sacrificed and serial sections of arch of aorta were harvested and processed for electron microscopic studies (EM). Results: EM studies showed thickened tunica intima, fenestration of internal elastic lamina and migration of smooth muscle cells from tunica media to tunica intima in the ovariectomized control, 1HSO and 5HSO treated groups, with the latter being most prominent. There were no significant ultra structural changes in the curcumin-treated groups compared to the non-treated groups. Conclusion: Oral administration of curcumin at a dose of 50 mg/ kg body weight did not show any changes in the aorta of the ovariectomized rats fed with 2% cholesterol and heated soy oil.

Original languageEnglish
Pages (from-to)307-313
Number of pages7
JournalClinica Terapeutica
Volume164
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Curcumin
Electrons
Oils
Ovariectomy
Tunica Intima
Atherosclerosis
Cholesterol
Tunica Media
Oil A
Thoracic Aorta
Vitamin E
Smooth Muscle Myocytes
Oral Administration
Aorta
Estrogens
Body Weight
Diet
Control Groups
Incidence

Keywords

  • Atherosclerosis
  • Curcumin
  • Electron microscope
  • Heated
  • Oestrogen
  • Ovariectomy
  • Soy oil

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{c1125b507aba432597b8388d5938baa8,
title = "Effect of curcumin on aortic changes in ovariectomized rats fed with repeatedly heated soy oil: A preliminary electron microscopic study",
abstract = "Background and aims: Consumption of repeatedly heated soy oil has been linked with incidence of atherosclerosis particularly in oestrogen deficient states. In the present study, effect of curcumin extract on the prevention of atherosclerosis was evaluated. Materials and Methods: Forty eight female Spraque-Dawley rats (weighing 200-250 gm) were divided into eight groups. All groups were fed with 2{\%} cholesterol diet. The sham control groups consisted of vitamin E free-RBD Olein (IV62) that acted as vehicle, and curcumin treated groups without undergoing ovariectomy. The other six groups were subjected to ovariectomy and later treated with vehicle-only, curcumin-only, once heated soy oil (1HSO) with vehicle, 1HSO with curcumin, five times heated soy oil (5HSO) with vehicle and 5HSO with curcumin. Curcumin was administered orally at a dose of 50 mg/kg which was commenced two weeks following ovariectomy. Following four months, the rats were sacrificed and serial sections of arch of aorta were harvested and processed for electron microscopic studies (EM). Results: EM studies showed thickened tunica intima, fenestration of internal elastic lamina and migration of smooth muscle cells from tunica media to tunica intima in the ovariectomized control, 1HSO and 5HSO treated groups, with the latter being most prominent. There were no significant ultra structural changes in the curcumin-treated groups compared to the non-treated groups. Conclusion: Oral administration of curcumin at a dose of 50 mg/ kg body weight did not show any changes in the aorta of the ovariectomized rats fed with 2{\%} cholesterol and heated soy oil.",
keywords = "Atherosclerosis, Curcumin, Electron microscope, Heated, Oestrogen, Ovariectomy, Soy oil",
author = "Jusoh, {A. Rashid} and Srijit Das and Kamsiah Jaarin and {Mohd Saad}, Qodriyah and Faizah Othman",
year = "2013",
doi = "10.7417/CT.2013.1578",
language = "English",
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pages = "307--313",
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T1 - Effect of curcumin on aortic changes in ovariectomized rats fed with repeatedly heated soy oil

T2 - A preliminary electron microscopic study

AU - Jusoh, A. Rashid

AU - Das, Srijit

AU - Jaarin, Kamsiah

AU - Mohd Saad, Qodriyah

AU - Othman, Faizah

PY - 2013

Y1 - 2013

N2 - Background and aims: Consumption of repeatedly heated soy oil has been linked with incidence of atherosclerosis particularly in oestrogen deficient states. In the present study, effect of curcumin extract on the prevention of atherosclerosis was evaluated. Materials and Methods: Forty eight female Spraque-Dawley rats (weighing 200-250 gm) were divided into eight groups. All groups were fed with 2% cholesterol diet. The sham control groups consisted of vitamin E free-RBD Olein (IV62) that acted as vehicle, and curcumin treated groups without undergoing ovariectomy. The other six groups were subjected to ovariectomy and later treated with vehicle-only, curcumin-only, once heated soy oil (1HSO) with vehicle, 1HSO with curcumin, five times heated soy oil (5HSO) with vehicle and 5HSO with curcumin. Curcumin was administered orally at a dose of 50 mg/kg which was commenced two weeks following ovariectomy. Following four months, the rats were sacrificed and serial sections of arch of aorta were harvested and processed for electron microscopic studies (EM). Results: EM studies showed thickened tunica intima, fenestration of internal elastic lamina and migration of smooth muscle cells from tunica media to tunica intima in the ovariectomized control, 1HSO and 5HSO treated groups, with the latter being most prominent. There were no significant ultra structural changes in the curcumin-treated groups compared to the non-treated groups. Conclusion: Oral administration of curcumin at a dose of 50 mg/ kg body weight did not show any changes in the aorta of the ovariectomized rats fed with 2% cholesterol and heated soy oil.

AB - Background and aims: Consumption of repeatedly heated soy oil has been linked with incidence of atherosclerosis particularly in oestrogen deficient states. In the present study, effect of curcumin extract on the prevention of atherosclerosis was evaluated. Materials and Methods: Forty eight female Spraque-Dawley rats (weighing 200-250 gm) were divided into eight groups. All groups were fed with 2% cholesterol diet. The sham control groups consisted of vitamin E free-RBD Olein (IV62) that acted as vehicle, and curcumin treated groups without undergoing ovariectomy. The other six groups were subjected to ovariectomy and later treated with vehicle-only, curcumin-only, once heated soy oil (1HSO) with vehicle, 1HSO with curcumin, five times heated soy oil (5HSO) with vehicle and 5HSO with curcumin. Curcumin was administered orally at a dose of 50 mg/kg which was commenced two weeks following ovariectomy. Following four months, the rats were sacrificed and serial sections of arch of aorta were harvested and processed for electron microscopic studies (EM). Results: EM studies showed thickened tunica intima, fenestration of internal elastic lamina and migration of smooth muscle cells from tunica media to tunica intima in the ovariectomized control, 1HSO and 5HSO treated groups, with the latter being most prominent. There were no significant ultra structural changes in the curcumin-treated groups compared to the non-treated groups. Conclusion: Oral administration of curcumin at a dose of 50 mg/ kg body weight did not show any changes in the aorta of the ovariectomized rats fed with 2% cholesterol and heated soy oil.

KW - Atherosclerosis

KW - Curcumin

KW - Electron microscope

KW - Heated

KW - Oestrogen

KW - Ovariectomy

KW - Soy oil

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DO - 10.7417/CT.2013.1578

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