Effect of compatibilizers on in vitro biocompatibility of PLA–HA bioscaffold

Sheik Mohideen Mohamed Nainar, Shahida Begum, M. N M Ansari, Md Enamul Hoque, S. Sharen Aini, Min Hwei Ng, Ruszymah Idrus

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

This paper exclusively describes the biocompatibility evaluation of biodegradable PLA–HA-based composites as temporary bone scaffolds for bone tissue engineering in orthopaedic applications. For that purpose, a set of composites were prepared using 3D melt-deposition method that comprises a biopolymer namely polylactic acid (PLA), and a bioceramic filler, namely hydroxyapatite (HA) 10 wt%, and compatibilizers, namely poly acrylic acid (PAA) 2 wt% and maleic anhydirde (MAH) 2 wt%. The composite samples were evaluated by in vitro assays and biodegradability tests were conducted in phosphate-buffered saline (PBS). For the in vitro analysis, osteogenic-induced stem cells were seeded onto the composite scaffold. An inverted optical microscope with computerised image analysis system was used to obtain data regarding cell attachment and contact characteristics after seeding for 48 h. Results showed that the PLA–HA-based composites did not induce adverse reactions from the cells, which in addition to their bone-matching mechanical properties makes them promising materials for bone scaffold applications.

Original languageEnglish
Pages (from-to)208-216
Number of pages9
JournalBioinspired, Biomimetic and Nanobiomaterials
Volume3
Issue number4
DOIs
Publication statusPublished - 1 Dec 2014

Fingerprint

Compatibilizers
Biocompatibility
Bone
Composite materials
carbopol 940
Scaffolds
Bioceramics
Biopolymers
Acids
Biodegradability
Orthopedics
Durapatite
Scaffolds (biology)
Stem cells
Hydroxyapatite
Tissue engineering
Image analysis
Acrylics
Fillers
Assays

Keywords

  • Biocompatibility
  • Bone scaffold
  • HA
  • Maleic anhydride (MAH)
  • PLA
  • Poly (acrylic acid) (PAA)

ASJC Scopus subject areas

  • Biomaterials
  • Engineering(all)

Cite this

Effect of compatibilizers on in vitro biocompatibility of PLA–HA bioscaffold. / Nainar, Sheik Mohideen Mohamed; Begum, Shahida; Ansari, M. N M; Hoque, Md Enamul; Aini, S. Sharen; Ng, Min Hwei; Idrus, Ruszymah.

In: Bioinspired, Biomimetic and Nanobiomaterials, Vol. 3, No. 4, 01.12.2014, p. 208-216.

Research output: Contribution to journalArticle

Nainar, Sheik Mohideen Mohamed ; Begum, Shahida ; Ansari, M. N M ; Hoque, Md Enamul ; Aini, S. Sharen ; Ng, Min Hwei ; Idrus, Ruszymah. / Effect of compatibilizers on in vitro biocompatibility of PLA–HA bioscaffold. In: Bioinspired, Biomimetic and Nanobiomaterials. 2014 ; Vol. 3, No. 4. pp. 208-216.
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