Effect of Allium sativum (Garlic) methanol extract on viability and apoptosis of human leukemic cell lines

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Abstract

Purpose: To investigate the effect of Allium sativum (garlic) methanol extract on viability and apoptosis of human leukemic cells. Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 ug/mL of Allium sativum extract following 48-h treatment on U-937, Jurkat Clone E6-1 and K-562 cell lines. The mode of cell death was determined by Annexin V-FITC staining and analyzed by flow cytometry. Results: The results show that the half-maximal inhibitory concentration (IC50) of A. sativum on U-937, Jurkat Clone E6-1, K-562 cell lines was 105 ± 2.21, 489 ± 4.51 and 455 ± 3.13 µg/mL, respectively, compared with negative control, while apoptosis was 17.93 ± 0.95 % for U-937 cells (p ≤ 0.05), 38.37 ± 1.88 % for Jurkat Clone E6-1 cells (p ≤ 0.001) and 16.37 ± 1.10 % for K-562 cells. A majority of the cells were inhibited by the extract via apoptosis. Only U-937 cells (6.87 ± 0.65 %) showed significant necrosis compared to negative control (p ≤ 0.05). Conclusion: K-562 cells are the most resistant against garlic extract, in contrast to Jurkat Clone E6-1 cells. Garlic extract does not induce necrosis in Jurkat Clone E6-1 and K-562 cells.

Original languageEnglish
Pages (from-to)1479-1485
Number of pages7
JournalTropical Journal of Pharmaceutical Research
Volume15
Issue number7
DOIs
Publication statusPublished - 1 Jul 2016

Fingerprint

Garlic
Methanol
Apoptosis
Cell Line
Clone Cells
Necrosis
Fluorescein-5-isothiocyanate
Annexin A5
Inhibitory Concentration 50
Cell Survival
Flow Cytometry
Cell Death
Staining and Labeling

Keywords

  • Allium sativum
  • Annexin V-FITC staining
  • Anti-leukemic
  • Apoptosis
  • Flow cytometry
  • Garlic
  • Jurkat Clone E6-1 cells
  • Necrosis

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

@article{563ce197b89140caa77dd11f21fe49c1,
title = "Effect of Allium sativum (Garlic) methanol extract on viability and apoptosis of human leukemic cell lines",
abstract = "Purpose: To investigate the effect of Allium sativum (garlic) methanol extract on viability and apoptosis of human leukemic cells. Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 ug/mL of Allium sativum extract following 48-h treatment on U-937, Jurkat Clone E6-1 and K-562 cell lines. The mode of cell death was determined by Annexin V-FITC staining and analyzed by flow cytometry. Results: The results show that the half-maximal inhibitory concentration (IC50) of A. sativum on U-937, Jurkat Clone E6-1, K-562 cell lines was 105 ± 2.21, 489 ± 4.51 and 455 ± 3.13 µg/mL, respectively, compared with negative control, while apoptosis was 17.93 ± 0.95 {\%} for U-937 cells (p ≤ 0.05), 38.37 ± 1.88 {\%} for Jurkat Clone E6-1 cells (p ≤ 0.001) and 16.37 ± 1.10 {\%} for K-562 cells. A majority of the cells were inhibited by the extract via apoptosis. Only U-937 cells (6.87 ± 0.65 {\%}) showed significant necrosis compared to negative control (p ≤ 0.05). Conclusion: K-562 cells are the most resistant against garlic extract, in contrast to Jurkat Clone E6-1 cells. Garlic extract does not induce necrosis in Jurkat Clone E6-1 and K-562 cells.",
keywords = "Allium sativum, Annexin V-FITC staining, Anti-leukemic, Apoptosis, Flow cytometry, Garlic, Jurkat Clone E6-1 cells, Necrosis",
author = "Malina Jasamai and Hui, {Chee Sze} and Norazrina Azmi and Endang, {Kumolosasi Msi}",
year = "2016",
month = "7",
day = "1",
doi = "10.4314/tjpr.v15i7.18",
language = "English",
volume = "15",
pages = "1479--1485",
journal = "Tropical Journal of Pharmaceutical Research",
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TY - JOUR

T1 - Effect of Allium sativum (Garlic) methanol extract on viability and apoptosis of human leukemic cell lines

AU - Jasamai, Malina

AU - Hui, Chee Sze

AU - Azmi, Norazrina

AU - Endang, Kumolosasi Msi

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Purpose: To investigate the effect of Allium sativum (garlic) methanol extract on viability and apoptosis of human leukemic cells. Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 ug/mL of Allium sativum extract following 48-h treatment on U-937, Jurkat Clone E6-1 and K-562 cell lines. The mode of cell death was determined by Annexin V-FITC staining and analyzed by flow cytometry. Results: The results show that the half-maximal inhibitory concentration (IC50) of A. sativum on U-937, Jurkat Clone E6-1, K-562 cell lines was 105 ± 2.21, 489 ± 4.51 and 455 ± 3.13 µg/mL, respectively, compared with negative control, while apoptosis was 17.93 ± 0.95 % for U-937 cells (p ≤ 0.05), 38.37 ± 1.88 % for Jurkat Clone E6-1 cells (p ≤ 0.001) and 16.37 ± 1.10 % for K-562 cells. A majority of the cells were inhibited by the extract via apoptosis. Only U-937 cells (6.87 ± 0.65 %) showed significant necrosis compared to negative control (p ≤ 0.05). Conclusion: K-562 cells are the most resistant against garlic extract, in contrast to Jurkat Clone E6-1 cells. Garlic extract does not induce necrosis in Jurkat Clone E6-1 and K-562 cells.

AB - Purpose: To investigate the effect of Allium sativum (garlic) methanol extract on viability and apoptosis of human leukemic cells. Methods: Cell viability was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay at concentrations of 3.125, 6.25, 12.5, 25, 50, 100, 200, 400 and 800 ug/mL of Allium sativum extract following 48-h treatment on U-937, Jurkat Clone E6-1 and K-562 cell lines. The mode of cell death was determined by Annexin V-FITC staining and analyzed by flow cytometry. Results: The results show that the half-maximal inhibitory concentration (IC50) of A. sativum on U-937, Jurkat Clone E6-1, K-562 cell lines was 105 ± 2.21, 489 ± 4.51 and 455 ± 3.13 µg/mL, respectively, compared with negative control, while apoptosis was 17.93 ± 0.95 % for U-937 cells (p ≤ 0.05), 38.37 ± 1.88 % for Jurkat Clone E6-1 cells (p ≤ 0.001) and 16.37 ± 1.10 % for K-562 cells. A majority of the cells were inhibited by the extract via apoptosis. Only U-937 cells (6.87 ± 0.65 %) showed significant necrosis compared to negative control (p ≤ 0.05). Conclusion: K-562 cells are the most resistant against garlic extract, in contrast to Jurkat Clone E6-1 cells. Garlic extract does not induce necrosis in Jurkat Clone E6-1 and K-562 cells.

KW - Allium sativum

KW - Annexin V-FITC staining

KW - Anti-leukemic

KW - Apoptosis

KW - Flow cytometry

KW - Garlic

KW - Jurkat Clone E6-1 cells

KW - Necrosis

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U2 - 10.4314/tjpr.v15i7.18

DO - 10.4314/tjpr.v15i7.18

M3 - Article

VL - 15

SP - 1479

EP - 1485

JO - Tropical Journal of Pharmaceutical Research

JF - Tropical Journal of Pharmaceutical Research

SN - 1596-5996

IS - 7

ER -