Effect of acacia honey on cultured rabbit corneal keratocytes

Choy Ker-Woon, Norzana Abd. Ghafar, Chua Kien Hui, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Acacia honey is a natural product which has proven to have therapeutic effects on skin wound healing, but its potential healing effects in corneal wound healing have not been studied. This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. Keratocytes from the corneal stroma of six New Zealand white rabbits were isolated and cultured until passage 1. The optimal dose of AH in the basal medium (FD) and medium containing serum (FDS) for keratocytes proliferation was identified using MTT assay. The morphological changes, gene and protein expressions of aldehyde dehydrogenase (ALDH), marker for quiescent keratocytes and vimentin, marker for fibroblasts were detected using q-RTPCR and immunocytochemistry respectively. Flowcytometry was performed to evaluate the cell cycle analysis of corneal keratocytes. Results: Cultured keratocytes supplemented with AH showed no morphological changes compared to control. Keratocytes cultured in FD and FDS media supplemented with 0.025% AH showed optimal proliferative potential compared with FD and FDS media, respectively. Gene expressions of ADLH and vimentin were increased in keratocytes cultured with AH enriched media. All proteins were expressed in keratocytes cultured in all media in accordance to the gene expression findings. No chromosomal changes were detected in keratocytes in AH enriched media.Conclusion: Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. The results of the present study show promising role of AH role in accelerating the initial stage of corneal wound healing.

Original languageEnglish
Article number19
JournalBMC Cell Biology
Volume15
Issue number1
DOIs
Publication statusPublished - 26 May 2014

Fingerprint

Corneal Keratocytes
Acacia
Honey
Rabbits
Wound Healing
Gene Expression
Vimentin
Cell Cycle
Proteins
Corneal Stroma
cdc Genes
Cell Cycle Proteins
Aldehyde Dehydrogenase
Therapeutic Uses
Biological Products
Fibroblasts
Immunohistochemistry

Keywords

  • Acacia honey
  • Corneal keratocytes
  • Proliferation

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Effect of acacia honey on cultured rabbit corneal keratocytes. / Ker-Woon, Choy; Abd. Ghafar, Norzana; Kien Hui, Chua; Mohd Yusof, Yasmin Anum.

In: BMC Cell Biology, Vol. 15, No. 1, 19, 26.05.2014.

Research output: Contribution to journalArticle

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