Kesan ß-gliserofosfat terhadap ampaian sel mononukleus daripada darah periferi manusia

Translated title of the contribution: Effect of ß-glycerophosphate on suspension mononucleus cell from human peripheral blood

Nurul Atikah Ahmad, Shahrul Hisham Zainal Ariffin, Rohaya Megat Abdul Wahab, Muhamad Abdul Razak, Sahidan Senafi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Osteoblast cell is a mononucleus cell that is responsible for bone formation. Mononucleus cell have been proven to differentiate into osteoblast cell after being induced with combinations of ascorbic acid and β-glycerophosphate as differentiation factors. This study aimed to observe the in vitro effect of a single β-glycerophosphate induction towards primary mononucleated cells suspension that have been isolated from human peripheral blood. Mononucleus cells were isolated from human peripheral blood using density gradient centrifugation method in the present of Ficoll-Paque Plus. Mononucleus cells were cultured for 7 days in proliferation medium before being induced by I mM, 5 mM, 10 mM, 20 mM, and 100 mM of β-glycerophosphate. Alkaline phosphatase (ALP) activity profile and morphological analysis were done at day 0, 1, 3, 7 and 14 in their respective medium. The activity of ALP enzyme and morphology analysis showed a significant increase (p<0.05) between cells that have been induced with the negative control cells by using paired t-test statistical analysis. In conclusion, the presence of only β-glycerophosphate is able to induce mononucleated cell differentiation into osteoblast cells. The effect of β-glycerophosphate towards mononucleated cells differentiation showed similar enzymological (ALP activities) and morphological profile as positif control (ascorbic acid and β-glycerophosphate). Based on enzymological and morphological analyses, 1 mM of β-glycerophosphate is the best concentration for in vitro osteoblast differentiation.

Original languageUndefined/Unknown
Pages (from-to)613-621
Number of pages9
JournalSains Malaysiana
Volume40
Issue number6
Publication statusPublished - Jun 2011

Fingerprint

Glycerophosphates
Suspensions
Osteoblasts
Alkaline Phosphatase
Ascorbic Acid
Cell Differentiation
Ficoll
Density Gradient Centrifugation
Osteogenesis
Cultured Cells

Keywords

  • β-Glycerophosphate
  • Suspension mononucleus cell

ASJC Scopus subject areas

  • General

Cite this

Kesan ß-gliserofosfat terhadap ampaian sel mononukleus daripada darah periferi manusia. / Ahmad, Nurul Atikah; Zainal Ariffin, Shahrul Hisham; Megat Abdul Wahab, Rohaya; Abdul Razak, Muhamad; Senafi, Sahidan.

In: Sains Malaysiana, Vol. 40, No. 6, 06.2011, p. 613-621.

Research output: Contribution to journalArticle

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