Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: In vitro study

Chua Kien Hui, Ting Hun Lee, Kamini Nagandran, Nor Hamdan Mohamad Yahaya, Chew Tin Lee, Eddie Tan Ti Tjih, Ramlan Abdul Aziz

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.

Original languageEnglish
Article number19
JournalBMC Complementary and Alternative Medicine
Volume13
DOIs
Publication statusPublished - 22 Jan 2013

Fingerprint

Protective Agents
Chondrocytes
Birds
Knee
Joints
Osteoarthritis
Cartilage
A73025
Knee Joint
Interleukin-8
Interleukin-1
Gene Expression
Dinoprostone
Interleukin-6
Aggrecans
Collagen Type II
In Vitro Techniques
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Matrix Metalloproteinases

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee : In vitro study. / Kien Hui, Chua; Lee, Ting Hun; Nagandran, Kamini; Mohamad Yahaya, Nor Hamdan; Lee, Chew Tin; Tjih, Eddie Tan Ti; Abdul Aziz, Ramlan.

In: BMC Complementary and Alternative Medicine, Vol. 13, 19, 22.01.2013.

Research output: Contribution to journalArticle

@article{51ec29a6be34415b9e33a44c6322668d,
title = "Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee: In vitro study",
abstract = "Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50{\%} - 1.00{\%} HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.",
author = "{Kien Hui}, Chua and Lee, {Ting Hun} and Kamini Nagandran and {Mohamad Yahaya}, {Nor Hamdan} and Lee, {Chew Tin} and Tjih, {Eddie Tan Ti} and {Abdul Aziz}, Ramlan",
year = "2013",
month = "1",
day = "22",
doi = "10.1186/1472-6882-13-19",
language = "English",
volume = "13",
journal = "BMC Complementary and Alternative Medicine",
issn = "1472-6882",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Edible Bird's nest extract as a chondro-protective agent for human chondrocytes isolated from osteoarthritic knee

T2 - In vitro study

AU - Kien Hui, Chua

AU - Lee, Ting Hun

AU - Nagandran, Kamini

AU - Mohamad Yahaya, Nor Hamdan

AU - Lee, Chew Tin

AU - Tjih, Eddie Tan Ti

AU - Abdul Aziz, Ramlan

PY - 2013/1/22

Y1 - 2013/1/22

N2 - Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.

AB - Background: Osteoarthritis (OA) is a degenerative joint disease that results in the destruction of cartilage. Edible Bird's Nest (EBN) extract contains important components, which can reduce the progression of osteoarthritis and helps in the regeneration of the cartilage. The present study aimed to investigate the effect of EBN extract on the catabolic and anabolic activities of the human articular chondrocytes (HACs) isolated from the knee joint of patients with OA.Methods: A single batch of EBN extract was prepared with hot-water extraction and coded as HMG. HACs were isolated from the knee joint cartilage removed during surgery. The optimum concentration of HMG for HAC cultures was determined using MTT assay. The effect of HMG on the catabolic and anabolic genes' expression in HACs was measured by real-time PCR. The total amount of prostaglandin E2 (PGE2) production was determined by ELISA method, and the total sulphated glycosaminoglycan (GAGs) production was quantified by 1,9-dimethylmethylene blue (DMMB) assay.Results: MTT assay showed 0.50% - 1.00% HMG supplementation promoted HACs proliferation. HMG supplementation was able to reduce the catabolic genes' expression in cultured HACs such as matrix metalloproteinases (MMP1 & MMP3), Interleukin 1, 6 and 8 (IL-1, IL-6 & IL-8), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Prostaglandin E2 (PGE2) production was significantly reduced in HAC cultures supplemented with HMG. With regard to anabolic activity assessment, type II collagen, Aggrecan and SOX-9 gene expression as well as sGAG production was increased in the HMG supplemented groups.Conclusion: Edible Bird's Nest extract coded as HMG demonstrated chondro-protection ability on human articular chondrocytes in vitro. It reduced catabolic activities and increased cartilage extracellular matrix synthesis. It is concluded that HMG is a potential agent in the treatment of osteoarthritis.

UR - http://www.scopus.com/inward/record.url?scp=84872446824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872446824&partnerID=8YFLogxK

U2 - 10.1186/1472-6882-13-19

DO - 10.1186/1472-6882-13-19

M3 - Article

C2 - 23339380

AN - SCOPUS:84872446824

VL - 13

JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

SN - 1472-6882

M1 - 19

ER -