Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time

M. Z. Mohd Waznul Adly, A. B. Zuraida, C. Y. Wee, Z. Alizah, A. B. Norliza, Che Radziah Che Mohd. Zain

Research output: Contribution to journalArticle

Abstract

Oil Palm Knotted-like 1(OPKN1) gene is a type of homeobox gene that was previously isolated from cDNA library of oil palm’s suspension cell culture. Based on semi-quantitative RT-PCR, northern blotting and in situ hybridization analysis, the OPKN1 gene is active in flower meristem suggesting its involvement in flowering phase. Semi-quantitative RT-PCR analysis indicated that OPKN1 gene is active in both male and female flowers and also in abnormal flower suggesting its role in flower morphogenesis process. To further validate the function of this gene, the overexpression transformation vector of OPKN1 gene driven by CaMV 35S constitutive promoter was constructed. The constructed vector was transformed into plant model systems Nicotiana benthamiana via Agrobacterium tumefaciens. Four transgenics lines of N. benthamiana were successfully obtained and analysis of quantitative Real-Time PCR shows that OPKN1 gene was highly overexpressed in all transgenic lines. Overexpression of OPKN1 gene in N. benthamiana transformant has caused reduction in N. benthamiana’s flower size and flowering time was also observed to be delayed. Based on this finding, it was suggested that OPKN1 may be involved in flower development and morphogenesis process.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalMalaysian Applied Biology
Volume44
Issue number1
Publication statusPublished - 2015

Fingerprint

Elaeis guineensis
Oils
flowering
flowers
Genes
Nicotiana benthamiana
genes
Morphogenesis
morphogenesis
reverse transcriptase polymerase chain reaction
genetically modified organisms
Polymerase Chain Reaction
Agrobacterium tumefaciens
gene overexpression
homeotic genes
male flowers
Meristem
female flowers
Ectopic Gene Expression
Homeobox Genes

Keywords

  • Gene expression
  • Knotted gene
  • N. benthamiana
  • Oil palm
  • Opkn1

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Mohd Waznul Adly, M. Z., Zuraida, A. B., Wee, C. Y., Alizah, Z., Norliza, A. B., & Che Mohd. Zain, C. R. (2015). Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time. Malaysian Applied Biology, 44(1), 101-106.

Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time. / Mohd Waznul Adly, M. Z.; Zuraida, A. B.; Wee, C. Y.; Alizah, Z.; Norliza, A. B.; Che Mohd. Zain, Che Radziah.

In: Malaysian Applied Biology, Vol. 44, No. 1, 2015, p. 101-106.

Research output: Contribution to journalArticle

Mohd Waznul Adly, MZ, Zuraida, AB, Wee, CY, Alizah, Z, Norliza, AB & Che Mohd. Zain, CR 2015, 'Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time', Malaysian Applied Biology, vol. 44, no. 1, pp. 101-106.
Mohd Waznul Adly, M. Z. ; Zuraida, A. B. ; Wee, C. Y. ; Alizah, Z. ; Norliza, A. B. ; Che Mohd. Zain, Che Radziah. / Ectopic expression of OPKN1 gene in N. benthamiana alter flower size and delay flowering time. In: Malaysian Applied Biology. 2015 ; Vol. 44, No. 1. pp. 101-106.
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