Dual-target detection using simultaneous amplification of PCR in clarifying interaction between Opiinae species (Hymenoptera: Braconidae) associated with Bactrocera spp. (Diptera: Tephritidae) infesting several crops

Salmah Yaakop, S. Shariff, N. J. Ibrahim, Badrul Munir Md. Zain, S. Yusof, N. Mohamad Jani

Research output: Contribution to journalArticle

Abstract

Simultaneous polymerase chain reaction (PCR) by using multiplex PCR was conducted to clarify the interaction between Opiinae associated with Bactroceracarambolae, B. papayae, and B cucurbitae. We sequenced and characterized a dual-band target detected on Opiinae DNA fragments by using two combination pairs of universal primers on two molecular markers, namely cytochrome oxidase subunit I (COI) and cytochrome b. Additionally, each Bactrocera species was identified by amplifying the COI region. Sequence data obtained from multiplex PCR seemed very effective in confirming species-level distinction that has been shown in tree topology. We conducted phylogenetic analyses prior to clarification of the interaction among three taxa levels. Interestingly, the sequences obtained from the simultaneous PCR successfully differentiated between six closely related Opiinae species in three genera, which could potentially be mass-reared as biocontrol agents of bactroceran fruit flies that infest several species of fruit. We discovered, proved, and added molecular data to clarify the interaction between Opiinae parasitoids, their host (Bactrocera spp.), and associated plants species. Psyttalia fletcheri parasitizing Bactrocera cucurbitae, which infests the ridge gourd fruit, has been added as a new record from Malaysia. This information would be extremely useful in taxonomic identification of species as part of an effective method in biological control program of the targeted fruit fly pests and their associated crops.

Original languageEnglish
Pages (from-to)121-131
Number of pages11
JournalArthropod-Plant Interactions
Volume9
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Bactrocera
Tephritidae
Braconidae
polymerase chain reaction
amplification
Hymenoptera
fruit
crop
cytochrome
crops
fruit flies
cytochrome-c oxidase
Psyttalia fletcheri
Luffa acutangula
Bactrocera cucurbitae
fruits
cytochrome b
biocontrol agent
topology
Malaysia

Keywords

  • Biological control
  • Cytochrome b
  • Cytochrome c oxidase subunit I
  • Fruit fly
  • Opiinae
  • Parasitoid

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Ecology
  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

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title = "Dual-target detection using simultaneous amplification of PCR in clarifying interaction between Opiinae species (Hymenoptera: Braconidae) associated with Bactrocera spp. (Diptera: Tephritidae) infesting several crops",
abstract = "Simultaneous polymerase chain reaction (PCR) by using multiplex PCR was conducted to clarify the interaction between Opiinae associated with Bactroceracarambolae, B. papayae, and B cucurbitae. We sequenced and characterized a dual-band target detected on Opiinae DNA fragments by using two combination pairs of universal primers on two molecular markers, namely cytochrome oxidase subunit I (COI) and cytochrome b. Additionally, each Bactrocera species was identified by amplifying the COI region. Sequence data obtained from multiplex PCR seemed very effective in confirming species-level distinction that has been shown in tree topology. We conducted phylogenetic analyses prior to clarification of the interaction among three taxa levels. Interestingly, the sequences obtained from the simultaneous PCR successfully differentiated between six closely related Opiinae species in three genera, which could potentially be mass-reared as biocontrol agents of bactroceran fruit flies that infest several species of fruit. We discovered, proved, and added molecular data to clarify the interaction between Opiinae parasitoids, their host (Bactrocera spp.), and associated plants species. Psyttalia fletcheri parasitizing Bactrocera cucurbitae, which infests the ridge gourd fruit, has been added as a new record from Malaysia. This information would be extremely useful in taxonomic identification of species as part of an effective method in biological control program of the targeted fruit fly pests and their associated crops.",
keywords = "Biological control, Cytochrome b, Cytochrome c oxidase subunit I, Fruit fly, Opiinae, Parasitoid",
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T2 - Braconidae) associated with Bactrocera spp. (Diptera: Tephritidae) infesting several crops

AU - Yaakop, Salmah

AU - Shariff, S.

AU - Ibrahim, N. J.

AU - Md. Zain, Badrul Munir

AU - Yusof, S.

AU - Mohamad Jani, N.

PY - 2015

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AB - Simultaneous polymerase chain reaction (PCR) by using multiplex PCR was conducted to clarify the interaction between Opiinae associated with Bactroceracarambolae, B. papayae, and B cucurbitae. We sequenced and characterized a dual-band target detected on Opiinae DNA fragments by using two combination pairs of universal primers on two molecular markers, namely cytochrome oxidase subunit I (COI) and cytochrome b. Additionally, each Bactrocera species was identified by amplifying the COI region. Sequence data obtained from multiplex PCR seemed very effective in confirming species-level distinction that has been shown in tree topology. We conducted phylogenetic analyses prior to clarification of the interaction among three taxa levels. Interestingly, the sequences obtained from the simultaneous PCR successfully differentiated between six closely related Opiinae species in three genera, which could potentially be mass-reared as biocontrol agents of bactroceran fruit flies that infest several species of fruit. We discovered, proved, and added molecular data to clarify the interaction between Opiinae parasitoids, their host (Bactrocera spp.), and associated plants species. Psyttalia fletcheri parasitizing Bactrocera cucurbitae, which infests the ridge gourd fruit, has been added as a new record from Malaysia. This information would be extremely useful in taxonomic identification of species as part of an effective method in biological control program of the targeted fruit fly pests and their associated crops.

KW - Biological control

KW - Cytochrome b

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