Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7,15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro

Nadiah Abu, Swee K. Yeap, Ahmad Z. Mat Pauzi, M. Nadeem Akhtar, Nur R. Zamberi, Jamil Ismail, Seema Zareen, Noorjahan B. Alitheen

Research output: Contribution to journalArticle

Abstract

The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.

Original languageEnglish
Article number89
JournalFrontiers in Pharmacology
Volume7
Issue numberMAR
DOIs
Publication statusPublished - 31 Mar 2016
Externally publishedYes

Fingerprint

HeLa Cells
Uterine Cervical Neoplasms
Cell Survival
Cell Death
Oligonucleotide Array Sequence Analysis
Flow Cytometry
Fritillaria
Genes
Afghanistan
Iraq
Protein Array Analysis
Thioredoxins
Pakistan
Heat-Shock Proteins
Oxidants
Superoxide Dismutase
Glutathione
Apoptosis
Gene Expression
Cell Line

Keywords

  • 15-dien-19-oic acid
  • Antioxidant
  • Fritillaria imperialis
  • HeLa
  • Isopimara-7

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7,15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro. / Abu, Nadiah; Yeap, Swee K.; Mat Pauzi, Ahmad Z.; Akhtar, M. Nadeem; Zamberi, Nur R.; Ismail, Jamil; Zareen, Seema; Alitheen, Noorjahan B.

In: Frontiers in Pharmacology, Vol. 7, No. MAR, 89, 31.03.2016.

Research output: Contribution to journalArticle

Abu, Nadiah ; Yeap, Swee K. ; Mat Pauzi, Ahmad Z. ; Akhtar, M. Nadeem ; Zamberi, Nur R. ; Ismail, Jamil ; Zareen, Seema ; Alitheen, Noorjahan B. / Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7,15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro. In: Frontiers in Pharmacology. 2016 ; Vol. 7, No. MAR.
@article{4835144f750d41b58b4424684f08e393,
title = "Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7,15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro",
abstract = "The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.",
keywords = "15-dien-19-oic acid, Antioxidant, Fritillaria imperialis, HeLa, Isopimara-7",
author = "Nadiah Abu and Yeap, {Swee K.} and {Mat Pauzi}, {Ahmad Z.} and Akhtar, {M. Nadeem} and Zamberi, {Nur R.} and Jamil Ismail and Seema Zareen and Alitheen, {Noorjahan B.}",
year = "2016",
month = "3",
day = "31",
doi = "10.3389/fphar.2016.00089",
language = "English",
volume = "7",
journal = "Frontiers in Pharmacology",
issn = "1663-9812",
publisher = "Frontiers Media S. A.",
number = "MAR",

}

TY - JOUR

T1 - Dual regulation of cell death and cell survival upon induction of cellular stress by Isopimara-7,15-Dien-19-Oic acid in cervical cancer, HeLa cells in vitro

AU - Abu, Nadiah

AU - Yeap, Swee K.

AU - Mat Pauzi, Ahmad Z.

AU - Akhtar, M. Nadeem

AU - Zamberi, Nur R.

AU - Ismail, Jamil

AU - Zareen, Seema

AU - Alitheen, Noorjahan B.

PY - 2016/3/31

Y1 - 2016/3/31

N2 - The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.

AB - The Fritillaria imperialis is an ornamental flower that can be found in various parts of the world including Iraq, Afghanistan, Pakistan, and the Himalayas. The use of this plant as traditional remedy is widely known. This study aims to unveil the anti-cancer potentials of Isopimara-7,15-Dien-19-Oic Acid, extracted from the bulbs of F. imperialis in cervical cancer cell line, HeLa cells. Flow cytometry analysis of cell death, gene expression analysis via cDNA microarray and protein array were performed. Based on the results, Isopimara-7,15-Dien-19-Oic acid simultaneously induced cell death and promoted cell survival. The execution of apoptosis was apparent based on the flow cytometry results and regulation of both pro and anti-apoptotic genes. Additionally, the regulation of anti-oxidant genes were up-regulated especially thioredoxin, glutathione and superoxide dismutase- related genes. Moreover, the treatment also induced the activation of pro-survival heat shock proteins. Collectively, Isopimara-7,15-Dien-19-Oic Acid managed to induce cellular stress in HeLa cells and activate several anti- and pro survival pathways.

KW - 15-dien-19-oic acid

KW - Antioxidant

KW - Fritillaria imperialis

KW - HeLa

KW - Isopimara-7

UR - http://www.scopus.com/inward/record.url?scp=84973102477&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973102477&partnerID=8YFLogxK

U2 - 10.3389/fphar.2016.00089

DO - 10.3389/fphar.2016.00089

M3 - Article

AN - SCOPUS:84973102477

VL - 7

JO - Frontiers in Pharmacology

JF - Frontiers in Pharmacology

SN - 1663-9812

IS - MAR

M1 - 89

ER -