Downregulation of toll-like receptor-mediated signalling pathways in oral lichen planus

Suraya Hani Mohd. Sinon, Alison M. Rich, Venkata P B Parachuru, Fiona A. Firth, Trudy Milne, Gregory J. Seymour

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: The objective of this study was to investigate the expression of Toll-like receptors (TLR) and TLR-associated signalling pathway genes in oral lichen planus (OLP). Methods: Initially, immunohistochemistry was used to determine TLR expression in 12 formalin-fixed archival OLP tissues with 12 non-specifically inflamed oral tissues as controls. RNA was isolated from further fresh samples of OLP and non-specifically inflamed oral tissue controls (n = 6 for both groups) and used in qRT2-PCR focused arrays to determine the expression of TLRs and associated signalling pathway genes. Genes with a statistical significance of ±two-fold regulation (FR) and a P-value <0.05 were considered as significantly regulated. Results: Significantly more TLR4+ cells were present in the inflammatory infiltrate in OLP compared with the control tissues (P <0.05). There was no statistically significant difference in the numbers of TLR2+ and TLR8+ cells between the groups. TLR3 was significantly downregulated in OLP (P <0.01). TLR8 was upregulated in OLP, but the difference between the groups was not statistically significant. The TLR-mediated signalling-associated protein genes MyD88 and TIRAP were significantly downregulated (P <0.01 and P <0.05), as were IRAK1 (P <0.05), MAPK8 (P <0.01), MAP3K1 (P <0.05), MAP4K4 (P <0.05), REL (P <0.01) and RELA (P <0.01). Stress proteins HMGB1 and the heat shock protein D1 were significantly downregulated in OLP (P <0.01). Conclusion: These findings suggest a downregulation of TLR-mediated signalling pathways in OLP lesions.

Original languageEnglish
Pages (from-to)28-34
Number of pages7
JournalJournal of Oral Pathology and Medicine
Volume45
Issue number1
DOIs
Publication statusPublished - 1 Jan 2016

Fingerprint

Oral Lichen Planus
Toll-Like Receptors
Down-Regulation
Heat-Shock Proteins
Myeloid Differentiation Factor 88
HMGB1 Protein
Genes
Formaldehyde
Immunohistochemistry
RNA
Polymerase Chain Reaction

Keywords

  • Oral lichen planus
  • Toll-like receptors

ASJC Scopus subject areas

  • Cancer Research
  • Pathology and Forensic Medicine
  • Otorhinolaryngology
  • Oral Surgery
  • Periodontics

Cite this

Downregulation of toll-like receptor-mediated signalling pathways in oral lichen planus. / Mohd. Sinon, Suraya Hani; Rich, Alison M.; Parachuru, Venkata P B; Firth, Fiona A.; Milne, Trudy; Seymour, Gregory J.

In: Journal of Oral Pathology and Medicine, Vol. 45, No. 1, 01.01.2016, p. 28-34.

Research output: Contribution to journalArticle

Mohd. Sinon, Suraya Hani ; Rich, Alison M. ; Parachuru, Venkata P B ; Firth, Fiona A. ; Milne, Trudy ; Seymour, Gregory J. / Downregulation of toll-like receptor-mediated signalling pathways in oral lichen planus. In: Journal of Oral Pathology and Medicine. 2016 ; Vol. 45, No. 1. pp. 28-34.
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