DOC and GCA blocks the reduction of hypothalamic CRF containing neurons due to repetitive stress

Farihah Suhaimi, Nabishah Mohamad, Khalid Bin Abdulkadir

Research output: Contribution to journalArticle

Abstract

Stress causes multiple adaptive hormonal responses including the secretion of CRF, ACTH and corticosteroids. These responses to repetitive stress could be blocked by naloxone, corticosteroids and glycyrrhizic acid (GCA). The effect of corticosteroids and GCA on the hypothalamic CRF containing neurons with repetitive stress has not been studied. Sprague-Dawley rats were given either dexamethasone (DEX), deoxycorticosterone (DOC) or GCA. On the eleventh day of treatment, the rats were exposed to two hours of immobilization stress and the procedure was repeated daily for another twelve days. The rats were sacrificed on days 2, 4, 8 and 12 after immobilization. On the days of sacrifice, the rats were transfused transcardially and the hypothalami were dissected for immunohistochemical analysis. With repetitive stress, the number of CRF containing neurons decreased significantly from 1631 ± 58 neurons/mm2 to 595 ± 71 neurons/mm2 (p<0.0005) on second exposure to stress and gradually increased to basal values 2058 ± 109 neurons/mm2 by the 8-12 exposures to stress. DOC and GCA completely inhibited this decrease in CRF containing neurons. DEX initially caused a significant decrease in CRF containing neurons compared to controls with repetitive stress. The release of CRF, which initiates the hypothalamus pituitary adrenal responses to stress, could be blocked by mineralocorticoids as well as glucocorticoids.

Original languageEnglish
Pages (from-to)17-20
Number of pages4
JournalNeuroanatomy
Volume6
Publication statusPublished - 2007

Fingerprint

Glycyrrhizic Acid
Desoxycorticosterone
Neurons
Adrenal Cortex Hormones
Immobilization
Dexamethasone
Hypothalamus
Mineralocorticoids
Corticotropin-Releasing Hormone
Naloxone
Glucocorticoids
Sprague Dawley Rats

Keywords

  • CRF containing neurons
  • Deoxycorticosterone
  • Dexamethasone
  • Glycyrrhizic acid
  • Paraventricular nucleus
  • Repetitive stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

DOC and GCA blocks the reduction of hypothalamic CRF containing neurons due to repetitive stress. / Suhaimi, Farihah; Mohamad, Nabishah; Abdulkadir, Khalid Bin.

In: Neuroanatomy, Vol. 6, 2007, p. 17-20.

Research output: Contribution to journalArticle

Suhaimi, Farihah ; Mohamad, Nabishah ; Abdulkadir, Khalid Bin. / DOC and GCA blocks the reduction of hypothalamic CRF containing neurons due to repetitive stress. In: Neuroanatomy. 2007 ; Vol. 6. pp. 17-20.
@article{3bb0577a0e554564b6040254acb2d2d8,
title = "DOC and GCA blocks the reduction of hypothalamic CRF containing neurons due to repetitive stress",
abstract = "Stress causes multiple adaptive hormonal responses including the secretion of CRF, ACTH and corticosteroids. These responses to repetitive stress could be blocked by naloxone, corticosteroids and glycyrrhizic acid (GCA). The effect of corticosteroids and GCA on the hypothalamic CRF containing neurons with repetitive stress has not been studied. Sprague-Dawley rats were given either dexamethasone (DEX), deoxycorticosterone (DOC) or GCA. On the eleventh day of treatment, the rats were exposed to two hours of immobilization stress and the procedure was repeated daily for another twelve days. The rats were sacrificed on days 2, 4, 8 and 12 after immobilization. On the days of sacrifice, the rats were transfused transcardially and the hypothalami were dissected for immunohistochemical analysis. With repetitive stress, the number of CRF containing neurons decreased significantly from 1631 ± 58 neurons/mm2 to 595 ± 71 neurons/mm2 (p<0.0005) on second exposure to stress and gradually increased to basal values 2058 ± 109 neurons/mm2 by the 8-12 exposures to stress. DOC and GCA completely inhibited this decrease in CRF containing neurons. DEX initially caused a significant decrease in CRF containing neurons compared to controls with repetitive stress. The release of CRF, which initiates the hypothalamus pituitary adrenal responses to stress, could be blocked by mineralocorticoids as well as glucocorticoids.",
keywords = "CRF containing neurons, Deoxycorticosterone, Dexamethasone, Glycyrrhizic acid, Paraventricular nucleus, Repetitive stress",
author = "Farihah Suhaimi and Nabishah Mohamad and Abdulkadir, {Khalid Bin}",
year = "2007",
language = "English",
volume = "6",
pages = "17--20",
journal = "Neuroanatomy",
issn = "1303-1783",
publisher = "Hacettepe University Faculty of Medicine",

}

TY - JOUR

T1 - DOC and GCA blocks the reduction of hypothalamic CRF containing neurons due to repetitive stress

AU - Suhaimi, Farihah

AU - Mohamad, Nabishah

AU - Abdulkadir, Khalid Bin

PY - 2007

Y1 - 2007

N2 - Stress causes multiple adaptive hormonal responses including the secretion of CRF, ACTH and corticosteroids. These responses to repetitive stress could be blocked by naloxone, corticosteroids and glycyrrhizic acid (GCA). The effect of corticosteroids and GCA on the hypothalamic CRF containing neurons with repetitive stress has not been studied. Sprague-Dawley rats were given either dexamethasone (DEX), deoxycorticosterone (DOC) or GCA. On the eleventh day of treatment, the rats were exposed to two hours of immobilization stress and the procedure was repeated daily for another twelve days. The rats were sacrificed on days 2, 4, 8 and 12 after immobilization. On the days of sacrifice, the rats were transfused transcardially and the hypothalami were dissected for immunohistochemical analysis. With repetitive stress, the number of CRF containing neurons decreased significantly from 1631 ± 58 neurons/mm2 to 595 ± 71 neurons/mm2 (p<0.0005) on second exposure to stress and gradually increased to basal values 2058 ± 109 neurons/mm2 by the 8-12 exposures to stress. DOC and GCA completely inhibited this decrease in CRF containing neurons. DEX initially caused a significant decrease in CRF containing neurons compared to controls with repetitive stress. The release of CRF, which initiates the hypothalamus pituitary adrenal responses to stress, could be blocked by mineralocorticoids as well as glucocorticoids.

AB - Stress causes multiple adaptive hormonal responses including the secretion of CRF, ACTH and corticosteroids. These responses to repetitive stress could be blocked by naloxone, corticosteroids and glycyrrhizic acid (GCA). The effect of corticosteroids and GCA on the hypothalamic CRF containing neurons with repetitive stress has not been studied. Sprague-Dawley rats were given either dexamethasone (DEX), deoxycorticosterone (DOC) or GCA. On the eleventh day of treatment, the rats were exposed to two hours of immobilization stress and the procedure was repeated daily for another twelve days. The rats were sacrificed on days 2, 4, 8 and 12 after immobilization. On the days of sacrifice, the rats were transfused transcardially and the hypothalami were dissected for immunohistochemical analysis. With repetitive stress, the number of CRF containing neurons decreased significantly from 1631 ± 58 neurons/mm2 to 595 ± 71 neurons/mm2 (p<0.0005) on second exposure to stress and gradually increased to basal values 2058 ± 109 neurons/mm2 by the 8-12 exposures to stress. DOC and GCA completely inhibited this decrease in CRF containing neurons. DEX initially caused a significant decrease in CRF containing neurons compared to controls with repetitive stress. The release of CRF, which initiates the hypothalamus pituitary adrenal responses to stress, could be blocked by mineralocorticoids as well as glucocorticoids.

KW - CRF containing neurons

KW - Deoxycorticosterone

KW - Dexamethasone

KW - Glycyrrhizic acid

KW - Paraventricular nucleus

KW - Repetitive stress

UR - http://www.scopus.com/inward/record.url?scp=40749088503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=40749088503&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:40749088503

VL - 6

SP - 17

EP - 20

JO - Neuroanatomy

JF - Neuroanatomy

SN - 1303-1783

ER -