DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode

Shafiquzzaman Siddiquee, Nor Azah Yusof, Abu Bakar Salleh, Soon Guan Tan, Fatimah Abu Bakar, Yook Heng Lee

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Electrochemical DNA sensors based on the recognition of a base single- or double-stranded DNA (ss/ds-DNA) sequence specific to a Trichoderma harzianum gene and immobilized onto a gold disk electrode are described here. The ssDNA probe was immobilized on the modified gold electrode to measure the electrochemical response. The hybridization of the probe ssDNA with the target DNA was explored by differential pulse voltammetry (DPV) using methylene blue (MB) as an electroactive indicator. MB on a modified gold electrode was found to be more largely localized than that on a bare gold electrode. Peak currents were found to increase in the order of hybrid-modified-AuE > probe-modified-AuE > non-harzianum > non-complementary DNA. Control experiments with the non-complementary oligonucleotides were performed to assess whether the DNA biosensor responded selectively, via hybridization, to the target. The variation in redox current with the different concentrations of target DNA was linear in the range of 1-20 ppm. Various properties and characteristics of this sensor were also described in this report. A novel approaches to construct an electrochemical biosensor consisting of the probe ssDNA to hybridize with crude DNA fragments from real samples was successfully applied in the study of biological microorganisms.

Original languageEnglish
Pages (from-to)198-205
Number of pages8
JournalSensors and Actuators, B: Chemical
Volume147
Issue number1
DOIs
Publication statusPublished - 18 May 2010

Fingerprint

Self assembled monolayers
immobilization
Gold
genes
DNA
deoxyribonucleic acid
Genes
gold
Electrodes
electrodes
probes
Methylene Blue
Biosensors
methylene blue
bioinstrumentation
Oligonucleotides
DNA sequences
Sensors
Voltammetry
oligonucleotides

Keywords

  • DNA
  • Electrochemical labeled probe
  • Hybridization
  • Trichoderma harzianum

ASJC Scopus subject areas

  • Instrumentation
  • Materials Chemistry
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering

Cite this

DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode. / Siddiquee, Shafiquzzaman; Yusof, Nor Azah; Salleh, Abu Bakar; Tan, Soon Guan; Bakar, Fatimah Abu; Lee, Yook Heng.

In: Sensors and Actuators, B: Chemical, Vol. 147, No. 1, 18.05.2010, p. 198-205.

Research output: Contribution to journalArticle

Siddiquee, Shafiquzzaman ; Yusof, Nor Azah ; Salleh, Abu Bakar ; Tan, Soon Guan ; Bakar, Fatimah Abu ; Lee, Yook Heng. / DNA hybridization based on Trichoderma harzianum gene probe immobilization on self-assembled monolayers on a modified gold electrode. In: Sensors and Actuators, B: Chemical. 2010 ; Vol. 147, No. 1. pp. 198-205.
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