DNA fragmentation into 200-250 and/or 30-50 kilobase pair fragments in rat liver nuclei is stimulated by Mg2+ alone and Ca2+/Mg2+ but not by Ca2+ alone

Kelvin Cain, Salmaan H. Inayat-Hussain, James T. Wolfe, Gerald M. Cohen

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Internucleosomal cleavage of DNA has often been regarded as the biochemical hallmark of apoptosis. We now demonstrate in isolated rat liver nuclei that DNA is initially cleaved into ≥700, 200-250 kbp and 30-50 kbp fragments via a multi-step process, which is activated by Mg+ and Mg2++Ca2+ but not by Ca2+ alone. The subsequent internucleosomal cleavage requires both cations. These findings demonstrate that a key event in the apoptotic process is the fragmentation of DNA into large kbp fragments by either a Mg2+ -dependent process (which can be potentiated by Ca2+) and/or by a Ca2+/Mg2+ activated endonuclease(s).

Original languageEnglish
Pages (from-to)385-391
Number of pages7
JournalFEBS Letters
Volume349
Issue number3
DOIs
Publication statusPublished - 8 Aug 1994
Externally publishedYes

Fingerprint

DNA Cleavage
Endonucleases
DNA Fragmentation
Liver
Cations
Rats
Apoptosis
DNA

Keywords

  • Apoptosis
  • DNA fragmentation
  • Endonuclease
  • Magnesium

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

DNA fragmentation into 200-250 and/or 30-50 kilobase pair fragments in rat liver nuclei is stimulated by Mg2+ alone and Ca2+/Mg2+ but not by Ca2+ alone. / Cain, Kelvin; Inayat-Hussain, Salmaan H.; Wolfe, James T.; Cohen, Gerald M.

In: FEBS Letters, Vol. 349, No. 3, 08.08.1994, p. 385-391.

Research output: Contribution to journalArticle

Cain, Kelvin ; Inayat-Hussain, Salmaan H. ; Wolfe, James T. ; Cohen, Gerald M. / DNA fragmentation into 200-250 and/or 30-50 kilobase pair fragments in rat liver nuclei is stimulated by Mg2+ alone and Ca2+/Mg2+ but not by Ca2+ alone. In: FEBS Letters. 1994 ; Vol. 349, No. 3. pp. 385-391.
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