DNA fingerprinting of septicemic and localized Burkholderia pseudomallei isolates from Malaysian patients

Mohd Noor Azura, A. Norazah, Mohamed Kamel Abdul Ghani, Ahmad Zorin Sahalan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We have analysed DNA fingerprinting patterns by pulsed-field gel electrophoresis (PFGE) of 52 unrelated Burkholderia pseudomallei strains isolated from septicemic and localized infections from Malaysian subjects. A total of 38 PFGE types were observed among 36 septicemic and 16 localized strains with no predominant pattern. Type 25 was seen in 2 epidemiologically related strains, suggesting human to human transmission. Twelve PFGE types were shared among 26 strains (21 septicemic and 5 localized) showing close genetic relatedness with coefficient of similarity of 0.81 to 1.0. The other 26 strains (15 septicemic and 11 localized) were unrelated as shown by the similarity coefficient of <0.8. This study showed that our B. pseudomallei strains in Malaysia were mainly heterogenous with no predominant type both in septicemic or localized strains.

Original languageEnglish
Pages (from-to)114-121
Number of pages8
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume42
Issue number1
Publication statusPublished - Jan 2011

Fingerprint

Burkholderia pseudomallei
DNA Fingerprinting
Pulsed Field Gel Electrophoresis
Malaysia
Infection

Keywords

  • Burkholderia pseudomallei
  • DNA fingerprinting
  • Malaysia
  • Melioidosis
  • PFGE

ASJC Scopus subject areas

  • Infectious Diseases
  • Public Health, Environmental and Occupational Health

Cite this

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abstract = "We have analysed DNA fingerprinting patterns by pulsed-field gel electrophoresis (PFGE) of 52 unrelated Burkholderia pseudomallei strains isolated from septicemic and localized infections from Malaysian subjects. A total of 38 PFGE types were observed among 36 septicemic and 16 localized strains with no predominant pattern. Type 25 was seen in 2 epidemiologically related strains, suggesting human to human transmission. Twelve PFGE types were shared among 26 strains (21 septicemic and 5 localized) showing close genetic relatedness with coefficient of similarity of 0.81 to 1.0. The other 26 strains (15 septicemic and 11 localized) were unrelated as shown by the similarity coefficient of <0.8. This study showed that our B. pseudomallei strains in Malaysia were mainly heterogenous with no predominant type both in septicemic or localized strains.",
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