Diversity of bacteria associated with the benthic marine dinoflagellates Coolia monotis and Ostreopsis ovata from Malaysian waters

Wong Weng Ruh, Asmat Ahmad, Mohd Noor Mat Isa, Nor Muhammad Mahadi, Noor Asyikin Marasan, Gires Usup

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The diversity of bacteria in two clonal cultures of the marine benthic dinoflagellates Coolia monotis and Ostreopsis ovata were assessed using molecular and culturing methods. Two 16S rDNA libraries were constructed from the directly amplified genomic DNAof bacteria associated with these dinoflagellates. Selective clones from different RFLP group were sequenced and analyzed. A total of 36 phylotypes was obtained from the directly amplified DNA of both cultures, and nine bacterial isolates were cultured from the C. monotis culture. A total of 205 sequences was obtained from the 16S rDNA libraries and these could be assigned to the taxa α-proteobacteria (44%), y-proteobacteria (27%), Cytophaga-Flavobacteria-Bacteroides (27%) and others (2%). The occurrence of common phylotypes between the two dinoflagellate cultures suggested species specification in the bacteria-dinoflagellate association. Only four culturable bacteria isolates were found in the metagenome 16S rDNA sequences of C. monotis culture. Culture-independent method is necessary to capture the full diversity of these associated bacteria since a large percentage (44%) of uncultured bacteria sequences were obtained.

Original languageEnglish
Pages (from-to)23-33
Number of pages11
JournalJournal of Science and Technology in the Tropics
Volume5
Issue number1
Publication statusPublished - Jun 2009

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Bacteria
Water
DNA
Specifications
Proteobacteria

Keywords

  • 16S rrna sequence analysis
  • Bacterial diversity
  • Benthic dinoflagellate
  • Dinoflagellate-culture associated bacteria

ASJC Scopus subject areas

  • General
  • Engineering(all)

Cite this

Diversity of bacteria associated with the benthic marine dinoflagellates Coolia monotis and Ostreopsis ovata from Malaysian waters. / Ruh, Wong Weng; Ahmad, Asmat; Isa, Mohd Noor Mat; Mahadi, Nor Muhammad; Marasan, Noor Asyikin; Usup, Gires.

In: Journal of Science and Technology in the Tropics, Vol. 5, No. 1, 06.2009, p. 23-33.

Research output: Contribution to journalArticle

Ruh, Wong Weng ; Ahmad, Asmat ; Isa, Mohd Noor Mat ; Mahadi, Nor Muhammad ; Marasan, Noor Asyikin ; Usup, Gires. / Diversity of bacteria associated with the benthic marine dinoflagellates Coolia monotis and Ostreopsis ovata from Malaysian waters. In: Journal of Science and Technology in the Tropics. 2009 ; Vol. 5, No. 1. pp. 23-33.
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