Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp.

Nadzirah Damiri, Hirzahida Mohd-Padil, Mohd Firdaus Mohd Raih

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The small RNA (sRNA) gene candidate, BpCand697 was previously reported to be unique to Burkholderia spp. and is encoded at 3′ non-coding region of a putative AraC family transcription regulator gene. This study demonstrates the conservation of BpCand697 sequence across 32 Burkholderia spp. including B. pseudomallei, B. mallei, B. thailandensis and Burkholderia sp. by integrating both sequence homology and secondary structural analyses of BpCand697 within the dataset. The divergent sequence of BpCand697 was also used as a discriminatory power in clustering the dataset according to the potential virulence of Burkholderia spp., showing that B. thailandensis was clearly secluded from the virulent cluster of B. pseudomallei and B. mallei. Finally, the differential co-transcript expression of BpCand697 and its flanking gene, bpsl2391 was detected in Burkholderia pseudomallei D286 after grown under two different culture conditions using nutrient-rich and minimal media. It is hypothesized that the differential expression of BpCand697-bpsl2391 co-transcript between the two standard prepared media might correlate with nutrient availability in the culture media, suggesting that the physical co-localization of BpCand697 in B. pseudomallei D286 might be directly or indirectly involved with the transcript regulation of bpsl2391 under the selected in vitro culture conditions.

Original languageEnglish
Title of host publication2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1678
ISBN (Electronic)9780735413252
DOIs
Publication statusPublished - 25 Sep 2015
Event2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015 - Selangor, Malaysia
Duration: 15 Apr 201516 Apr 2015

Other

Other2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015
CountryMalaysia
CitySelangor
Period15/4/1516/4/15

Fingerprint

genes
nutrients
virulence
culture media
regulators
homology
availability
conservation

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Damiri, N., Mohd-Padil, H., & Mohd Raih, M. F. (2015). Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium (Vol. 1678). [030008] American Institute of Physics Inc.. https://doi.org/10.1063/1.4931229

Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp. / Damiri, Nadzirah; Mohd-Padil, Hirzahida; Mohd Raih, Mohd Firdaus.

2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015. 030008.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Damiri, N, Mohd-Padil, H & Mohd Raih, MF 2015, Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp. in 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. vol. 1678, 030008, American Institute of Physics Inc., 2015 Postgraduate Colloquium of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology, UKM FST 2015, Selangor, Malaysia, 15/4/15. https://doi.org/10.1063/1.4931229
Damiri N, Mohd-Padil H, Mohd Raih MF. Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp. In 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678. American Institute of Physics Inc. 2015. 030008 https://doi.org/10.1063/1.4931229
Damiri, Nadzirah ; Mohd-Padil, Hirzahida ; Mohd Raih, Mohd Firdaus. / Divergent homologs of the predicted small RNA BpCand697 in Burkholderia spp. 2015 UKM FST Postgraduate Colloquium: Proceedings of the Universiti Kebangsaan Malaysia, Faculty of Science and Technology 2015 Postgraduate Colloquium. Vol. 1678 American Institute of Physics Inc., 2015.
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