Divalent CATIONS (Mg2+, Ca2+) protect bacterial outer membrane damage by polymyxin B

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Polymyxin B interacts with divalent cations by displacing cations from their binding sites in the lipopolysaccharide (LPS) molecules. It leads to the disorganization of the outer membrane component of the Gram negative bacteria, which releases LPS component from bacterial surface causing severe membrane leakage and finally cell death. In this paper, the reversible activity of PMB was investigated in the presence of access divalent cations such as Mg 2+ or Ca2+. Membrane damage and cell disruption were monitored by detecting leakage of the outer membrane and cytoplasmic enzyme marker (β-lactamase and β-galactosidase), release of LPS component (KDO) from bacterial cells and bacterial survival. With the presence of divalent cations, leakage of enzyme markers and LPS released was significantly reduced when bacteria were exposed to PMB. The survival curve also significantly increased. The inhibitory of damage caused by PMB also depended on the type of divalent cation present. Ca2+ has been shown to be more effective in protecting the bacteria cells than Mg2+. This is because Ca 2+ appears more frequently as a constituent of the structural components of the bacteria. In conclusion, the presence of higher concentration of divalent cation particularly with Ca2+ inhibits PMB activity and maintained bacterial survival.

Original languageEnglish
Pages (from-to)301-306
Number of pages6
JournalSains Malaysiana
Volume42
Issue number3
Publication statusPublished - Mar 2013

Fingerprint

Polymyxin B
Divalent Cations
Lipopolysaccharides
Membranes
Bacteria
Cell Membrane
Galactosidases
Enzymes
Gram-Negative Bacteria
Cations
Cell Survival
Cell Death
Binding Sites

Keywords

  • Calcium
  • Divalent cation
  • E.coli
  • Magnesium
  • Polymyxin B

ASJC Scopus subject areas

  • General

Cite this

Divalent CATIONS (Mg2+, Ca2+) protect bacterial outer membrane damage by polymyxin B. / Sahalan, Ahmad Zorin; Abdul, Abdul Hamid; Hing, Hiang Lian; Abdul Ghani, Mohamed Kamel.

In: Sains Malaysiana, Vol. 42, No. 3, 03.2013, p. 301-306.

Research output: Contribution to journalArticle

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AB - Polymyxin B interacts with divalent cations by displacing cations from their binding sites in the lipopolysaccharide (LPS) molecules. It leads to the disorganization of the outer membrane component of the Gram negative bacteria, which releases LPS component from bacterial surface causing severe membrane leakage and finally cell death. In this paper, the reversible activity of PMB was investigated in the presence of access divalent cations such as Mg 2+ or Ca2+. Membrane damage and cell disruption were monitored by detecting leakage of the outer membrane and cytoplasmic enzyme marker (β-lactamase and β-galactosidase), release of LPS component (KDO) from bacterial cells and bacterial survival. With the presence of divalent cations, leakage of enzyme markers and LPS released was significantly reduced when bacteria were exposed to PMB. The survival curve also significantly increased. The inhibitory of damage caused by PMB also depended on the type of divalent cation present. Ca2+ has been shown to be more effective in protecting the bacteria cells than Mg2+. This is because Ca 2+ appears more frequently as a constituent of the structural components of the bacteria. In conclusion, the presence of higher concentration of divalent cation particularly with Ca2+ inhibits PMB activity and maintained bacterial survival.

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