Distribution of bacteriophages in food and environment samples

J. M.K.J.K. Premarathne, T. Y. Thung, C. Y. New, J. T.Y. Huat, Dayang Fredalina Basri, Y. Rukayadi, Y. Nakaguchi, M. Nishibuchi, R. Son

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Foodborne pathogens have become a constant threat to the consumer and food industry. Reduce efficacy of antibiotics with emergence of resistant bacteria has limited the opportunities for controlling pathogenic bacteria in food commodities and treating foodborne infections. Bacteriophages can be a promising alternative for alleviate the risk of transmitting pathogenic bacteria via food commodities. Therefore, this research was conducted to find distribution of bacteriophages in diverse niches in order to identify suitable sources for isolating bacteriophages to use controlling foodborne pathogens. Firstly bacterial strains were screened for lysogenic and selected suitable host bacterial strains were used for isolating and determining bacteriophage titer in fresh raw food and environmental samples. Eighteen different lytic bacteriophages effective against Campylobacter, S. aureus, L. monocytogenes and E. coli were isolated from this study. Bacteriophages titer was determined within range of 102 to 1010 PFU/mL and bacteriophages were most frequently isolated from chicken (60%) samples. The isolated bacteriophages could be potential candidates for controlling foodborne diseases.

Original languageEnglish
Pages (from-to)888-896
Number of pages9
JournalInternational Food Research Journal
Volume24
Issue number2
Publication statusPublished - 2017

Fingerprint

bacteriophages
Bacteriophages
Food
commodity foods
sampling
raw foods
food pathogens
foodborne illness
Bacteria
bacteria
Foodborne Diseases
Campylobacter
Food Industry
food industry
Chickens
niches
antibiotics
Escherichia coli
chickens
Anti-Bacterial Agents

Keywords

  • Bacteriophage titer
  • Bacteriophages
  • Double layer assay
  • Foodborne pathogens
  • Plaques

ASJC Scopus subject areas

  • Food Science

Cite this

Premarathne, J. M. K. J. K., Thung, T. Y., New, C. Y., Huat, J. T. Y., Basri, D. F., Rukayadi, Y., ... Son, R. (2017). Distribution of bacteriophages in food and environment samples. International Food Research Journal, 24(2), 888-896.

Distribution of bacteriophages in food and environment samples. / Premarathne, J. M.K.J.K.; Thung, T. Y.; New, C. Y.; Huat, J. T.Y.; Basri, Dayang Fredalina; Rukayadi, Y.; Nakaguchi, Y.; Nishibuchi, M.; Son, R.

In: International Food Research Journal, Vol. 24, No. 2, 2017, p. 888-896.

Research output: Contribution to journalArticle

Premarathne, JMKJK, Thung, TY, New, CY, Huat, JTY, Basri, DF, Rukayadi, Y, Nakaguchi, Y, Nishibuchi, M & Son, R 2017, 'Distribution of bacteriophages in food and environment samples', International Food Research Journal, vol. 24, no. 2, pp. 888-896.
Premarathne JMKJK, Thung TY, New CY, Huat JTY, Basri DF, Rukayadi Y et al. Distribution of bacteriophages in food and environment samples. International Food Research Journal. 2017;24(2):888-896.
Premarathne, J. M.K.J.K. ; Thung, T. Y. ; New, C. Y. ; Huat, J. T.Y. ; Basri, Dayang Fredalina ; Rukayadi, Y. ; Nakaguchi, Y. ; Nishibuchi, M. ; Son, R. / Distribution of bacteriophages in food and environment samples. In: International Food Research Journal. 2017 ; Vol. 24, No. 2. pp. 888-896.
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