Distribution of 5-HT<inf>3</inf>, 5-HT<inf>4</inf>, and 5-HT<inf>7</inf> receptors along the human colon

Nor Syafinaz Yaakob, Kenneth A. Chinkwo, Navinisha Chetty, Ian M. Coupar, Helen R. Irving

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background/Aims: Several disorders of the gastrointestinal tract are associated with abnormal serotonin (5-HT) signaling or metabolism where the 5-HT<inf>3</inf> and 5-HT<inf>4</inf> receptors are clinically relevant. The aim was to examine the distribution of 5-HT<inf>3</inf>, 5-HT<inf>4</inf>, and 5-HT<inf>7</inf> receptors in the normal human colon and how this is associated with receptor interacting chaperone 3, G protein coupled receptor kinases, and protein LIN-7 homologs to extend previous observations limited to the sigmoid colon or the upper intestine. Methods: Samples from ascending, transverse, descending, and sigmoid human colon were dissected into 3 separate layers (mucosa, longitudinal, and circular muscles) and ileum samples were dissected into mucosa and muscle layers (n = 20). Complementary DNA was synthesized by reverse transcription from extracted RNA and expression was determined by quantitative or end point polymerase chain reaction. Results: The 5-HT<inf>3</inf> receptor subunits were found in all tissues throughout the colon and ileum. The A subunit was detected in all samples and the C subunit was expressed at similar levels while the B subunit was expressed at lower levels and less frequently. The 5-HT<inf>3</inf> receptor E subunit was mainly found in the mucosa layers. All splice variants of the 5-HT<inf>4</inf> and 5-HT<inf>7</inf> receptors were expressed throughout the colon although the 5-HT<inf>4</inf> receptor d, g, and i variants were expressed less often. Conclusions: The major differences in 5-HT receptor distribution within the human colon are in relation to the mucosa and muscular tissue layers where the 5-HT<inf>3</inf> receptor E subunit is predominantly found in the mucosal layer which may be of therapeutic relevance.

Original languageEnglish
Pages (from-to)361-369
Number of pages9
JournalJournal of Neurogastroenterology and Motility
Volume21
Issue number3
DOIs
Publication statusPublished - 2015

Fingerprint

Receptors, Serotonin, 5-HT3
Colon
Mucous Membrane
Receptors, Serotonin, 5-HT4
Sigmoid Colon
Ileum
G-Protein-Coupled Receptor Kinase 3
Serotonin
Muscles
Serotonin Receptors
Reverse Transcription
Intestines
Gastrointestinal Tract
Complementary DNA
RNA
Polymerase Chain Reaction
serotonin 7 receptor
Proteins
Therapeutics

Keywords

  • Colon
  • G protein coupled receptor kinases
  • Ileum
  • Receptors
  • Serotonin

ASJC Scopus subject areas

  • Gastroenterology
  • Clinical Neurology

Cite this

Distribution of 5-HT<inf>3</inf>, 5-HT<inf>4</inf>, and 5-HT<inf>7</inf> receptors along the human colon. / Yaakob, Nor Syafinaz; Chinkwo, Kenneth A.; Chetty, Navinisha; Coupar, Ian M.; Irving, Helen R.

In: Journal of Neurogastroenterology and Motility, Vol. 21, No. 3, 2015, p. 361-369.

Research output: Contribution to journalArticle

Yaakob, Nor Syafinaz ; Chinkwo, Kenneth A. ; Chetty, Navinisha ; Coupar, Ian M. ; Irving, Helen R. / Distribution of 5-HT<inf>3</inf>, 5-HT<inf>4</inf>, and 5-HT<inf>7</inf> receptors along the human colon. In: Journal of Neurogastroenterology and Motility. 2015 ; Vol. 21, No. 3. pp. 361-369.
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AU - Coupar, Ian M.

AU - Irving, Helen R.

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