Distribution and Characterization of Rhogocyte Cell Types in the Mantle Tissue of Haliotis laevigata

Mohd Fareed Mohd Sairi, Peter Valtchev, Vincent G. Gomes, Fariba Dehghani

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Molluscan rhogocytes are known to be the only cells able to synthesize hemocyanin that is one of the largest respiratory proteins in nature. However, investigation of rhogocyte cells in vitro is limited due to difficulty in isolating and establishing marine cell culture. The aim of this study was to investigate the nature and distribution of rhogocyte cells of Haliotis laevigata in the mantle tissue with respect to the expression of the two known isoforms of hemocyanin. Rhogocyte cells were identified using immunofluorescence-fluorescence in situ hybridization (IF-FISH) that involved simultaneous staining of localized hemocyanin by a polyclonal antibody while the mRNA was hybridized with FISH probes. The distribution of rhogocyte cells was demonstrated using flow cytometry, followed by cell sorting with fluorescence-activated cell sorter (FACS) and confocal microscope imaging for further characterization. Our results suggested that the mantle tissue is dominated by two distinct populations of rhogocyte cells that synthesize hemocyanin type 1. Observation with confocal microscopy of both populations revealed hemocyanin localization in the periphery of the cell membrane. Cell population with higher antibody signal had irregular and elongated cell morphology with punctate mRNA probe signals. The second population with lower antibody signal had ovoid morphology and wide distribution of mRNA probe signals. We suggest that these populations represent two distinct phases of hemocyanin biosynthesis of a single isoform, which is closely related to Haliotis tuberculata type 1 hemocyanin (HtH1). The knowledge acquired in this study enhances the understanding of the biology of rhogocyte cells and biosynthesis of hemocyanin.

Original languageEnglish
Pages (from-to)168-179
Number of pages12
JournalMarine Biotechnology
Volume17
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Haliotis laevigata
Hemocyanin
antibody
mantle
probe
fluorescence
cells
probes (equipment)
flow cytometry
Population
sorting
microscopy
Messenger RNA
Antibodies
Flow Cytometry
Protein Isoforms
membrane
Haliotis tuberculata
protein
tissues

Keywords

  • Fluorescence-activated cell sorting (FACS)
  • Hemocyanin
  • Immunofluorescence-fluorescence in situ hybridization (IF-FISH)
  • Mollusk
  • Rhogocyte cell

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Distribution and Characterization of Rhogocyte Cell Types in the Mantle Tissue of Haliotis laevigata. / Mohd Sairi, Mohd Fareed; Valtchev, Peter; Gomes, Vincent G.; Dehghani, Fariba.

In: Marine Biotechnology, Vol. 17, No. 2, 2015, p. 168-179.

Research output: Contribution to journalArticle

Mohd Sairi, Mohd Fareed ; Valtchev, Peter ; Gomes, Vincent G. ; Dehghani, Fariba. / Distribution and Characterization of Rhogocyte Cell Types in the Mantle Tissue of Haliotis laevigata. In: Marine Biotechnology. 2015 ; Vol. 17, No. 2. pp. 168-179.
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