Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene

Luigi De Bellis, Ismanizan Ismail, Susan J. Reynolds, Matthieu D. Barrett, Steven M. Smith

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Recent data have shown that distinct DNA sequence elements direct the germination and sugar responses of the cucumber malate synthase (Ms) gene. Such information is, however, lacking for the isocitrate lyase (Id) gene which is coordinately regulated with Ms. Deletions from the 5' end of the Icl promoter were therefore created specifically to address this question. Analysis of expression in seeds of transgenic Nicotiana plumbaginifolia plants showed that whereas a promoter sequence of 2.9 kilobases (kb) produced a normal germination response, deletion to -1568 base pairs (bp) dramatically reduced this response. Examination of the sugar response employed a transgenic cucumber root system. In this case, the 2900 bp and 1568 bp promoters both gave a strong sugar response, but further deletion to -1367 bp eliminated the response. Therefore, the germination and sugar responses of the Icl gene require distinct cis-acting elements, located respectively upstream and downstream of -1568 bp. This observation is consistent with distinct signal transduction systems regulating gene expression in each case.

Original languageEnglish
Pages (from-to)375-378
Number of pages4
JournalGene
Volume197
Issue number1-2
DOIs
Publication statusPublished - 15 Sep 1997
Externally publishedYes

Fingerprint

Isocitrate Lyase
Cucumis sativus
Germination
Base Pairing
Malate Synthase
Genes
Tobacco
Signal Transduction
Seeds
Gene Expression

Keywords

  • Cucumis sativus L
  • Gene expression
  • Glyoxylate cycle
  • Hairy roots
  • Seed germination
  • Transgenic plant

ASJC Scopus subject areas

  • Genetics

Cite this

Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene. / De Bellis, Luigi; Ismail, Ismanizan; Reynolds, Susan J.; Barrett, Matthieu D.; Smith, Steven M.

In: Gene, Vol. 197, No. 1-2, 15.09.1997, p. 375-378.

Research output: Contribution to journalArticle

De Bellis, Luigi ; Ismail, Ismanizan ; Reynolds, Susan J. ; Barrett, Matthieu D. ; Smith, Steven M. / Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene. In: Gene. 1997 ; Vol. 197, No. 1-2. pp. 375-378.
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