Dissimilatory Fe(III) reduction by an electrochemically active lactic acid bacterium phylogenetically related to Enterococcus gallinarum isolated from submerged soil

G. T. Kim, M. S. Hyun, I. S. Chang, H. J. Kim, H. S. Park, B. H. Kim, S. D. Kim, J. W T Wimpenny, Andrew J. Weightman

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Aims: The isolation and identification of a glucose-oxidizing Fe(III)-reducing bacteria (FRB) with electrochemical activity from an anoxic environment, and characterization of the role of Fe(III) in its metabolism. Methods and Results: A Gram-positive (Firmicutes), nonmotile, coccoid and facultative anaerobic FRB was isolated based on its ability to reduce Fe(III). Using the Vitek Gram-positive identification card kit and 16S rRNA gene sequence analysis, the isolate was identified as Enterococcus gallinarum, designated strain MG25. On glucose this isolate produced lactate plus small amounts of acetate, formate and CO2 and its growth rates were similar in the presence and absence of Fe(O)OH. These results suggest that MG25 can couple glucose oxidation to Fe(III) reduction, but without conservation of energy to support growth. Cyclic voltammetry showed that strain MG25 was electrochemically active. Conclusions: An electrochemically active and FRB, E. gallinarum MG25, was isolated from submerged soil. Fe(III) is used in the bacterial metabolism as an electron sink. Significance and Impact of the Study: This is the first report concerning the electrochemical activity of glucose-oxidizing FRB, E. gallinarum. This organism and others like it could be used as new biocatalysts to improve the performance of a mediator-less microbial fuel cell.

Original languageEnglish
Pages (from-to)978-987
Number of pages10
JournalJournal of Applied Microbiology
Volume99
Issue number4
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint

Enterococcus gallinarum
Enterococcus
lactic acid bacteria
Lactic Acid
Soil
Bacteria
Glucose
glucose
formic acid
soil
Bioelectric Energy Sources
microbial fuel cells
energy conservation
metabolism
Firmicutes
formates
Growth
rRNA Genes
lactates
Sequence Analysis

Keywords

  • Electrochemical activity
  • Electron sink
  • Enterococcus gallinarum
  • Fe(III)-reducing bacteria
  • Mediator-less microbial fuel cell

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

Dissimilatory Fe(III) reduction by an electrochemically active lactic acid bacterium phylogenetically related to Enterococcus gallinarum isolated from submerged soil. / Kim, G. T.; Hyun, M. S.; Chang, I. S.; Kim, H. J.; Park, H. S.; Kim, B. H.; Kim, S. D.; Wimpenny, J. W T; Weightman, Andrew J.

In: Journal of Applied Microbiology, Vol. 99, No. 4, 2005, p. 978-987.

Research output: Contribution to journalArticle

Kim, G. T. ; Hyun, M. S. ; Chang, I. S. ; Kim, H. J. ; Park, H. S. ; Kim, B. H. ; Kim, S. D. ; Wimpenny, J. W T ; Weightman, Andrew J. / Dissimilatory Fe(III) reduction by an electrochemically active lactic acid bacterium phylogenetically related to Enterococcus gallinarum isolated from submerged soil. In: Journal of Applied Microbiology. 2005 ; Vol. 99, No. 4. pp. 978-987.
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AU - Kim, G. T.

AU - Hyun, M. S.

AU - Chang, I. S.

AU - Kim, H. J.

AU - Park, H. S.

AU - Kim, B. H.

AU - Kim, S. D.

AU - Wimpenny, J. W T

AU - Weightman, Andrew J.

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AB - Aims: The isolation and identification of a glucose-oxidizing Fe(III)-reducing bacteria (FRB) with electrochemical activity from an anoxic environment, and characterization of the role of Fe(III) in its metabolism. Methods and Results: A Gram-positive (Firmicutes), nonmotile, coccoid and facultative anaerobic FRB was isolated based on its ability to reduce Fe(III). Using the Vitek Gram-positive identification card kit and 16S rRNA gene sequence analysis, the isolate was identified as Enterococcus gallinarum, designated strain MG25. On glucose this isolate produced lactate plus small amounts of acetate, formate and CO2 and its growth rates were similar in the presence and absence of Fe(O)OH. These results suggest that MG25 can couple glucose oxidation to Fe(III) reduction, but without conservation of energy to support growth. Cyclic voltammetry showed that strain MG25 was electrochemically active. Conclusions: An electrochemically active and FRB, E. gallinarum MG25, was isolated from submerged soil. Fe(III) is used in the bacterial metabolism as an electron sink. Significance and Impact of the Study: This is the first report concerning the electrochemical activity of glucose-oxidizing FRB, E. gallinarum. This organism and others like it could be used as new biocatalysts to improve the performance of a mediator-less microbial fuel cell.

KW - Electrochemical activity

KW - Electron sink

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KW - Fe(III)-reducing bacteria

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