Disruption of methicillin-resistant Staphylococcus aureus protein synthesis by tannins

Siti Noor Adnalizawati Adnan, Nazlina Ibrahim, Wan Yaacob Wan Ahmad

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Introduction Methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide public health threat, displaying multiple antibiotic resistance that causes morbidity and mortality. Management of multidrug-resistant (MDR) MRSA infections is extremely difficult due to their inherent resistance to currently used antibiotics. New antibiotics are needed to combat the emergence of antimicrobial resistance. Methods The in vitro effect of tannins was studied against MRSA reference strain (ATCC 43300) and MRSA clinical strains utilizing antimicrobial assays in conjunction with both scanning and transmission electron microscopy. To reveal the influence of tannins in MRSA protein synthesis disruption, we utilized next-generation sequencing (NGS) to provide further insight into the novel protein synthesis transcriptional response of MRSA exposed to these compounds. Results Tannins possessed both bacteriostatic and bactericidal activity with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 0.78 and 1.56 mg/mL, respectively, against all tested MRSA. Scanning and transmission electron microscopy of MRSA treated with tannins showed decrease in cellular volume, indicating disruption of protein synthesis. Conclusion Analysis of a genome-wide transcriptional profile of the reference strain ATCC 43300 MRSA in response to tannins has led to the finding that tannins induced significant modulation in essential ribosome pathways, which caused a reduction in the translation processes that lead to inhibition of protein synthesis and obviation of bacterial growth. These findings highlight the potential of tannins as new promising anti-MRSA agents in clinical application such as body wash and topical cream or ointments.

Original languageEnglish
Pages (from-to)186-192
Number of pages7
JournalGERMS
Volume7
Issue number4
DOIs
Publication statusPublished - 1 Dec 2017

Fingerprint

Tannins
Methicillin-Resistant Staphylococcus aureus
Proteins
Scanning Transmission Electron Microscopy
Anti-Bacterial Agents
Microbial Sensitivity Tests
Microbial Drug Resistance
Ointments
Ribosomes
Public Health
Genome
Morbidity
Mortality

Keywords

  • Antimicrobial assays
  • MRSA
  • Ribosomal protein synthesis
  • Tannins

ASJC Scopus subject areas

  • Epidemiology
  • Immunology and Microbiology(all)
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Disruption of methicillin-resistant Staphylococcus aureus protein synthesis by tannins. / Adnan, Siti Noor Adnalizawati; Ibrahim, Nazlina; Wan Ahmad, Wan Yaacob.

In: GERMS, Vol. 7, No. 4, 01.12.2017, p. 186-192.

Research output: Contribution to journalArticle

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