Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting

Noor Haslinda Noor Hashim, Amatul Hamizah Ali, Alfi Khatib, Jalifah Latip

Research output: Contribution to journalArticle

Abstract

Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.

Original languageEnglish
Article number102966
JournalVibrational Spectroscopy
Volume104
DOIs
Publication statusPublished - 1 Sep 2019

Fingerprint

sulfoxide
Sulfones
Sonication
Aromatic compounds
Terpenes
Medical problems
Glycosides
Bioactivity
Hydroxyl Radical
Functional groups
Quality control
Skin
Carbohydrates
Spectroscopy
Processing
Chemical analysis

Keywords

  • Antiplasmodial
  • Clinacanthus nutans
  • Fourier transform infrared spectroscopy (FTIR)
  • Metabolomics
  • Multivariate data analysis

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting. / Noor Hashim, Noor Haslinda; Ali, Amatul Hamizah; Khatib, Alfi; Latip, Jalifah.

In: Vibrational Spectroscopy, Vol. 104, 102966, 01.09.2019.

Research output: Contribution to journalArticle

@article{1f04aa5474424168ae8135870172f7b1,
title = "Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting",
abstract = "Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.",
keywords = "Antiplasmodial, Clinacanthus nutans, Fourier transform infrared spectroscopy (FTIR), Metabolomics, Multivariate data analysis",
author = "{Noor Hashim}, {Noor Haslinda} and Ali, {Amatul Hamizah} and Alfi Khatib and Jalifah Latip",
year = "2019",
month = "9",
day = "1",
doi = "10.1016/j.vibspec.2019.102966",
language = "English",
volume = "104",
journal = "Vibrational Spectroscopy",
issn = "0924-2031",
publisher = "Elsevier",

}

TY - JOUR

T1 - Discrimination of Clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting

AU - Noor Hashim, Noor Haslinda

AU - Ali, Amatul Hamizah

AU - Khatib, Alfi

AU - Latip, Jalifah

PY - 2019/9/1

Y1 - 2019/9/1

N2 - Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.

AB - Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.

KW - Antiplasmodial

KW - Clinacanthus nutans

KW - Fourier transform infrared spectroscopy (FTIR)

KW - Metabolomics

KW - Multivariate data analysis

UR - http://www.scopus.com/inward/record.url?scp=85071876638&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85071876638&partnerID=8YFLogxK

U2 - 10.1016/j.vibspec.2019.102966

DO - 10.1016/j.vibspec.2019.102966

M3 - Article

AN - SCOPUS:85071876638

VL - 104

JO - Vibrational Spectroscopy

JF - Vibrational Spectroscopy

SN - 0924-2031

M1 - 102966

ER -