Direct fermentation of gelatinized sago starch to acetone-butanol-ethanol by Clostridium acetobutylicum

M. S. Madihah, A. B. Ariff, Mohd. Sahaid Kalil, A. A. Suraini, M. I A Karim

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Direct fermentation of gelatinized sago starch into solvent (acetone-butanol-ethanol) by Clostridium acetobutylicum P262 was studied using a 250 ml Schott bottle anaerobic fermentation system. Total solvent production from fermentation using 30 g sago starch/l (11.03 g/l) was comparable to fermentation using corn starch and about 2-fold higher than fermentation using potato or tapioca starch. At the range of sago starch concentration investigated (10-80 g/l), the highest total solvent production (18.82 g/l) was obtained at 50 g/l. The use of a mixture of organic and inorganic nitrogen source (yeast extract + NH4NO3) enhanced growth of C. acetobutylicum, starch hydrolysis and solvent production (24.47 g/l) compared to the use of yeast extract alone. This gave the yield based on sugar consumed of 0.45 g/g. Result from this study also showed that the individual concentrations of nitrogen and carbon influenced solvent production to a greater extent than did carbon to nitrogen (C/N) ratio.

Original languageEnglish
Pages (from-to)567-576
Number of pages10
JournalWorld Journal of Microbiology and Biotechnology
Volume17
Issue number6
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Clostridium acetobutylicum
sago
Clostridium
Butanols
butanol
Acetone
Starch
Fermentation
acetone
Ethanol
ethanol
fermentation
yeast extract
Nitrogen
nitrogen
Yeast
starch
Carbon
tapioca
Yeasts

Keywords

  • Acetone
  • Butanol
  • C/N ratio
  • Clostridium acetobutylicum
  • Ethanol
  • Sago starch

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Plant Science
  • Biochemistry
  • Food Science
  • Microbiology

Cite this

Direct fermentation of gelatinized sago starch to acetone-butanol-ethanol by Clostridium acetobutylicum. / Madihah, M. S.; Ariff, A. B.; Kalil, Mohd. Sahaid; Suraini, A. A.; Karim, M. I A.

In: World Journal of Microbiology and Biotechnology, Vol. 17, No. 6, 2001, p. 567-576.

Research output: Contribution to journalArticle

@article{01be3bc77ce640089a3d7b7e05c11e0f,
title = "Direct fermentation of gelatinized sago starch to acetone-butanol-ethanol by Clostridium acetobutylicum",
abstract = "Direct fermentation of gelatinized sago starch into solvent (acetone-butanol-ethanol) by Clostridium acetobutylicum P262 was studied using a 250 ml Schott bottle anaerobic fermentation system. Total solvent production from fermentation using 30 g sago starch/l (11.03 g/l) was comparable to fermentation using corn starch and about 2-fold higher than fermentation using potato or tapioca starch. At the range of sago starch concentration investigated (10-80 g/l), the highest total solvent production (18.82 g/l) was obtained at 50 g/l. The use of a mixture of organic and inorganic nitrogen source (yeast extract + NH4NO3) enhanced growth of C. acetobutylicum, starch hydrolysis and solvent production (24.47 g/l) compared to the use of yeast extract alone. This gave the yield based on sugar consumed of 0.45 g/g. Result from this study also showed that the individual concentrations of nitrogen and carbon influenced solvent production to a greater extent than did carbon to nitrogen (C/N) ratio.",
keywords = "Acetone, Butanol, C/N ratio, Clostridium acetobutylicum, Ethanol, Sago starch",
author = "Madihah, {M. S.} and Ariff, {A. B.} and Kalil, {Mohd. Sahaid} and Suraini, {A. A.} and Karim, {M. I A}",
year = "2001",
doi = "10.1023/A:1012351112351",
language = "English",
volume = "17",
pages = "567--576",
journal = "World Journal of Microbiology and Biotechnology",
issn = "0959-3993",
publisher = "Springer Netherlands",
number = "6",

}

TY - JOUR

T1 - Direct fermentation of gelatinized sago starch to acetone-butanol-ethanol by Clostridium acetobutylicum

AU - Madihah, M. S.

AU - Ariff, A. B.

AU - Kalil, Mohd. Sahaid

AU - Suraini, A. A.

AU - Karim, M. I A

PY - 2001

Y1 - 2001

N2 - Direct fermentation of gelatinized sago starch into solvent (acetone-butanol-ethanol) by Clostridium acetobutylicum P262 was studied using a 250 ml Schott bottle anaerobic fermentation system. Total solvent production from fermentation using 30 g sago starch/l (11.03 g/l) was comparable to fermentation using corn starch and about 2-fold higher than fermentation using potato or tapioca starch. At the range of sago starch concentration investigated (10-80 g/l), the highest total solvent production (18.82 g/l) was obtained at 50 g/l. The use of a mixture of organic and inorganic nitrogen source (yeast extract + NH4NO3) enhanced growth of C. acetobutylicum, starch hydrolysis and solvent production (24.47 g/l) compared to the use of yeast extract alone. This gave the yield based on sugar consumed of 0.45 g/g. Result from this study also showed that the individual concentrations of nitrogen and carbon influenced solvent production to a greater extent than did carbon to nitrogen (C/N) ratio.

AB - Direct fermentation of gelatinized sago starch into solvent (acetone-butanol-ethanol) by Clostridium acetobutylicum P262 was studied using a 250 ml Schott bottle anaerobic fermentation system. Total solvent production from fermentation using 30 g sago starch/l (11.03 g/l) was comparable to fermentation using corn starch and about 2-fold higher than fermentation using potato or tapioca starch. At the range of sago starch concentration investigated (10-80 g/l), the highest total solvent production (18.82 g/l) was obtained at 50 g/l. The use of a mixture of organic and inorganic nitrogen source (yeast extract + NH4NO3) enhanced growth of C. acetobutylicum, starch hydrolysis and solvent production (24.47 g/l) compared to the use of yeast extract alone. This gave the yield based on sugar consumed of 0.45 g/g. Result from this study also showed that the individual concentrations of nitrogen and carbon influenced solvent production to a greater extent than did carbon to nitrogen (C/N) ratio.

KW - Acetone

KW - Butanol

KW - C/N ratio

KW - Clostridium acetobutylicum

KW - Ethanol

KW - Sago starch

UR - http://www.scopus.com/inward/record.url?scp=0035191049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035191049&partnerID=8YFLogxK

U2 - 10.1023/A:1012351112351

DO - 10.1023/A:1012351112351

M3 - Article

AN - SCOPUS:0035191049

VL - 17

SP - 567

EP - 576

JO - World Journal of Microbiology and Biotechnology

JF - World Journal of Microbiology and Biotechnology

SN - 0959-3993

IS - 6

ER -