Direct discrimination of different plant populations and study on temperature effects by Fourier transform infrared spectroscopy

Khairunisa Khairudin, Nur Afiqah Sukiran, Hoe Han Goh, Syarul Nataqain Baharum, Normah Mohd Noor

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two-dimensional correlation infrared spectroscopy (2D-IR) correlation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm-1 (hydroxyl group), 2,927 cm-1 (methyl group), 1,623 cm-1 (carbonyl group), and 1,068 cm-1 (C-O group) were particularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terpenes, amide and flavonoids were more intense for the highland populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm-1) and (1,634, 1,555 cm-1) in the 2D-IR correlation spectra provided fingerprint signals to differentiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discriminate different populations of P. minus and the effects of temperature.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalMetabolomics
DOIs
Publication statusAccepted/In press - 2013

Fingerprint

Fourier Transform Infrared Spectroscopy
Thermal effects
Temperature
Population
Perturbation techniques
Terpenes
Flavonoids
Amides
Hydroxyl Radical
Infrared spectroscopy
Polygonum
Carbohydrates
Controlled Environment
Dermatoglyphics
Spectrum Analysis
Hot Temperature

Keywords

  • Fourier transform infrared spectroscopy (FTIR)
  • Metabolite screening
  • Plant populations
  • Temperature effect
  • Two-dimensional correlation infrared spectroscopy (2D-IR)

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Clinical Biochemistry
  • Biochemistry

Cite this

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title = "Direct discrimination of different plant populations and study on temperature effects by Fourier transform infrared spectroscopy",
abstract = "Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two-dimensional correlation infrared spectroscopy (2D-IR) correlation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm-1 (hydroxyl group), 2,927 cm-1 (methyl group), 1,623 cm-1 (carbonyl group), and 1,068 cm-1 (C-O group) were particularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terpenes, amide and flavonoids were more intense for the highland populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm-1) and (1,634, 1,555 cm-1) in the 2D-IR correlation spectra provided fingerprint signals to differentiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discriminate different populations of P. minus and the effects of temperature.",
keywords = "Fourier transform infrared spectroscopy (FTIR), Metabolite screening, Plant populations, Temperature effect, Two-dimensional correlation infrared spectroscopy (2D-IR)",
author = "Khairunisa Khairudin and Sukiran, {Nur Afiqah} and Goh, {Hoe Han} and Baharum, {Syarul Nataqain} and Noor, {Normah Mohd}",
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AU - Khairudin, Khairunisa

AU - Sukiran, Nur Afiqah

AU - Goh, Hoe Han

AU - Baharum, Syarul Nataqain

AU - Noor, Normah Mohd

PY - 2013

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N2 - Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two-dimensional correlation infrared spectroscopy (2D-IR) correlation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm-1 (hydroxyl group), 2,927 cm-1 (methyl group), 1,623 cm-1 (carbonyl group), and 1,068 cm-1 (C-O group) were particularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terpenes, amide and flavonoids were more intense for the highland populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm-1) and (1,634, 1,555 cm-1) in the 2D-IR correlation spectra provided fingerprint signals to differentiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discriminate different populations of P. minus and the effects of temperature.

AB - Fourier transform infrared spectroscopy was used to characterise highland and lowland populations of Polygonum minus Huds. grown in different controlled environments. A thermal perturbation technique of two-dimensional correlation infrared spectroscopy (2D-IR) correlation spectra was applied to establish differences between the populations. The absorption peaks at 3,480 cm-1 (hydroxyl group), 2,927 cm-1 (methyl group), 1,623 cm-1 (carbonyl group), and 1,068 cm-1 (C-O group) were particularly powerful in separating the populations. These peaks, which indicate the presence of carbohydrate, terpenes, amide and flavonoids were more intense for the highland populations than lowland populations, and increased in environments with a higher temperature. Wavenumbers (1,634, 669 cm-1) and (1,634, 1,555 cm-1) in the 2D-IR correlation spectra provided fingerprint signals to differentiate plants grown at different temperatures. This study demonstrates that IR fingerprinting, which combines mid-IR spectra and 2D-IR correlation spectra, can directly discriminate different populations of P. minus and the effects of temperature.

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