Differentiation potential of human suspension mononucleated peripheral blood cells

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Stem cells are known to have the ability to renew themselves and differentiate into a diverse range of specialized cell types. Currently, the differentiation potential of human peripheral blood mononucleated cells in suspension as stem cells is not well-understood. The aim of this study was to investigate the differentiation potential of suspension mononucleated cells from human peripheral blood to differentiate. The osteoblast and osteoclast differentiation potential of suspension peripheral blood mononucleated cells were examined by molecular, biochemical and cell morphology analyses. The expression of osteoblast marker (osteonectin, SPARC) and osteoclast marker (tartrate resistant acid phosphatase, TRAP) as well as high alkaline phosphate (ALP) and TRAP enzyme activity were observed at days 14 and 10 of osteoblast and osteoclast differentiation, respectively. Morphology analyses showed that mononucleated cells successfully differentiated into osteoblasts and osteoclasts. The existence of stem cells in mononucleated cells was evaluated by the expression of a stem cell factor (KIT) and a haematopoietic stem cell marker (signalling lymphocytic activation molecule family member 1, SLAMF1). This study has shown that these suspension mononucleated cells possess differentiation potential through in vitro study. Human peripheral blood suspension mononucleated cells that have multi-lineage differentiation potential may provide a new source of stem cells that may be used for bone regeneration and tissue engineering applications.

Original languageEnglish
Pages (from-to)10756-10764
Number of pages9
JournalAfrican Journal of Biotechnology
Volume10
Issue number52
Publication statusPublished - 12 Sep 2011

Fingerprint

osteoclasts
osteoblasts
blood cells
stem cells
Blood Cells
Suspensions
Osteoclasts
cell suspension culture
Osteoblasts
Stem Cells
acid phosphatase
cells
stem cell factor
tissue engineering
blood
Osteonectin
cell differentiation
in vitro studies
Bone Regeneration
Stem Cell Factor

Keywords

  • Osteoblast cells
  • Osteoblast differentiation
  • Osteoclast cells
  • Osteoclast differentiation
  • Peripheral blood

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science

Cite this

Differentiation potential of human suspension mononucleated peripheral blood cells. / Kadir, Ruzanna Ab; Zainal Ariffin, Shahrul Hisham; Megat Abdul Wahab, Rohaya; Senafi, Sahidan; Huyop, Fahrul Zaman.

In: African Journal of Biotechnology, Vol. 10, No. 52, 12.09.2011, p. 10756-10764.

Research output: Contribution to journalArticle

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