Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model

Chee Yin Wong, Soon Keng Cheong, Pooi Ling Mok, Leong Chooi Fun

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Aims: Adult human bone marrow contains a population of mesenchymal stem cells (MSC) that contributes to the regeneration of tissues such as bone, cartilage, muscle, tendon, and fat. In recent years, it has been shown that functional stem cells exist in the adult bone marrow, and they can contribute to renal remodelling or reconstitution of injured renal glomeruli, especially mesangial cells. The purpose of this study is to examine the ability of MSC isolated from human bone marrow to differentiate into mesangial cells in glomerular injured athymic mice. Methods: MSC were isolated from human bone marrow mononuclear cells based on plastic adherent properties and expanded in vitro in the culture medium. Human mesenchymal stem cells (hMSC) were characterised using microscopy, immunophenotyping, and their ability to differentiate into adipocytes, chondrocytes, and osteocytes. hMSC were then injected into athymic mice, which had induced glomerulonephropathy (GN). Results: Test mice (induced GN and infused hMSC) were shown to have anti-human CD105+ cells present in the kidneys and were also positive to anti-human desmin, a marker for mesangial cells. Furthermore, immunofluorescence assays also demonstrated that anti-human desmin+ cells in the glomeruli of these test mice were in the proliferation stage, being positive to anti-human Ki-67. Conclusions: These findings indicate that hMSC found in renal glomeruli differentiated into mesangial cells in vivo after glomerular injury occurred.

Original languageEnglish
Pages (from-to)52-57
Number of pages6
JournalPathology
Volume40
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Mesangial Cells
Mesenchymal Stromal Cells
Wounds and Injuries
Desmin
Bone Marrow
Kidney
Nude Mice
Osteocytes
Immunophenotyping
Chondrocytes
Adipocytes
Bone Marrow Cells
Tendons
Plastics
Cartilage
Fluorescent Antibody Technique
Culture Media
Regeneration
Microscopy
Stem Cells

Keywords

  • Histopathology
  • In vivo differentiation
  • Mesangial cell
  • Mesenchymal stem cell
  • Renal

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model. / Wong, Chee Yin; Cheong, Soon Keng; Mok, Pooi Ling; Chooi Fun, Leong.

In: Pathology, Vol. 40, No. 1, 01.2008, p. 52-57.

Research output: Contribution to journalArticle

Wong, Chee Yin ; Cheong, Soon Keng ; Mok, Pooi Ling ; Chooi Fun, Leong. / Differentiation of human mesenchymal stem cells into mesangial cells in post-glomerular injury murine model. In: Pathology. 2008 ; Vol. 40, No. 1. pp. 52-57.
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