Differentiation of dental pulp stem cells into neuron-like cells in serum-free medium

Shahrul Hisham Zainal Ariffin, Shabnam Kermani, Intan Zarina Zainol Abidin, Rohaya Megat Abdul Wahab, Zulham Yamamoto, Sahidan Senafi, Zaidah Zainal Ariffin, Mohamad Abdul Razak

Research output: Contribution to journalArticle

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Abstract

Dental pulp tissue contains dental pulp stem cells (DPSCs). Dental pulp cells (also known as dental pulp-derived mesenchymal stem cells) are capable of differentiating into multilineage cells including neuron-like cells. The aim of this study was to examine the capability of DPSCs to differentiate into neuron-like cells without using any reagents or growth factors. DPSCs were isolated from teeth extracted from 6- to 8-week-old mice and maintained in complete medium. The cells from the fourth passage were induced to differentiate by culturing in medium without serum or growth factors. RT-PCR molecular analysis showed characteristics of Cd146+, Cd166+, and Cd31- in DPSCs, indicating that these cells are mesenchymal stem cells rather than hematopoietic stem cells. After 5 days of neuronal differentiation, the cells showed neuron-like morphological changes and expressed MAP2 protein. The activation of Nestin was observed at low level prior to differentiation and increased after 5 days of culture in differentiation medium, whereas Tub3 was activated only after 5 days of neuronal differentiation. The proliferation of the differentiated cells decreased in comparison to that of the control cells. Dental pulp stem cells are induced to differentiate into neuron-like cells when cultured in serum- and growth factor-free medium.

Original languageEnglish
Article number250740
JournalStem Cells International
DOIs
Publication statusPublished - 2013

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Dental Pulp
Serum-Free Culture Media
Stem Cells
Neurons
Intercellular Signaling Peptides and Proteins
Mesenchymal Stromal Cells
Nestin
Hematopoietic Stem Cells
Serum
Cell Differentiation
Cultured Cells
Tooth
Cell Proliferation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Differentiation of dental pulp stem cells into neuron-like cells in serum-free medium. / Zainal Ariffin, Shahrul Hisham; Kermani, Shabnam; Zainol Abidin, Intan Zarina; Megat Abdul Wahab, Rohaya; Yamamoto, Zulham; Senafi, Sahidan; Zainal Ariffin, Zaidah; Abdul Razak, Mohamad.

In: Stem Cells International, 2013.

Research output: Contribution to journalArticle

Zainal Ariffin, Shahrul Hisham ; Kermani, Shabnam ; Zainol Abidin, Intan Zarina ; Megat Abdul Wahab, Rohaya ; Yamamoto, Zulham ; Senafi, Sahidan ; Zainal Ariffin, Zaidah ; Abdul Razak, Mohamad. / Differentiation of dental pulp stem cells into neuron-like cells in serum-free medium. In: Stem Cells International. 2013.
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AU - Megat Abdul Wahab, Rohaya

AU - Yamamoto, Zulham

AU - Senafi, Sahidan

AU - Zainal Ariffin, Zaidah

AU - Abdul Razak, Mohamad

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