Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus

Shahrul Hisham Zainal Ariffin, Intan Zarina Zainol Abidin, Muhammad Dain Yazid, Rohaya Megat Abdul Wahab

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: The purpose of this study is to determine whether isolated suspension mouse peripheral mononucleated blood cells have the potential to differentiate into two distinct types of cells, i.e., osteoblasts and osteoclasts. Results: Differentiation into osteoblast cells was concomitant with the activation of the Opn gene, increment of alkaline phosphatase (ALP) activity and the existence of bone nodules, whereas osteoclast cells activated the Catk gene, increment of tartrate resistant acid phosphatase (TRAP) activity and showed resorption activities via resorption pits. Morphology analyses showed the morphology of osteoblast and osteoclast cells after von Kossa and May-Grunwald-Giemsa staining respectively. Conclusions: In conclusion, suspension mononucleated cells have the potentiality to differentiate into mature osteoblasts and osteoclasts, and hence can be categorized as multipotent stem cells.

Original languageEnglish
Article number29
JournalCell Communication and Signaling
Volume8
DOIs
Publication statusPublished - 2010

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Osteoblasts
Osteoclasts
Blood Cells
Suspensions
Blood
Cells
Genes
Multipotent Stem Cells
Acid Phosphatase
Stem cells
Alkaline Phosphatase
Bone
Chemical activation
Transcriptional Activation
Staining and Labeling
Bone and Bones

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Cite this

Differentiation analyses of adult suspension mononucleated peripheral blood cells of Mus musculus. / Zainal Ariffin, Shahrul Hisham; Abidin, Intan Zarina Zainol; Yazid, Muhammad Dain; Megat Abdul Wahab, Rohaya.

In: Cell Communication and Signaling, Vol. 8, 29, 2010.

Research output: Contribution to journalArticle

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