Differential proteomic analysis of a polymicrobial biofilm

Zamirah Zainal Abidin, Paul D. Veith, Stuart G. Dashper, Ying Zhu, Deanne V. Catmull, Yu Yen Chen, Deasy C. Heryanto, Dina Chen, James S. Pyke, Kheng Tan, Helen L. Mitchell, Eric C. Reynolds

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. The aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. In a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture P. gingivalis and T. denticola were closely associated and dominated the polymicrobial biofilm. For comparison, planktonic cultures of P. gingivalis and T. denticola were grown separately in continuous culture. Whole cell lysates were subjected to SDS-PAGE, followed by in-gel proteolytic H2 16O/H2 18O labeling. From two replicates, 135 and 174 P. gingivalis proteins and 134 and 194 T. denticola proteins were quantified by LC-MALDI TOF/TOF MS. The results suggest a change of strategy in iron acquisition by P. gingivalis due to large increases in the abundance of HusA and HusB in the polymicrobial biofilm while HmuY and other iron/haem transport systems decreased. Significant changes in the abundance of peptidases and enzymes involved in glutamate and glycine catabolism suggest syntrophy. These data indicate an intimate association between P. gingivalis and T. denticola in a biofilm that may play a role in disease pathogenesis.

Original languageEnglish
Pages (from-to)4449-4464
Number of pages16
JournalJournal of Proteome Research
Volume11
Issue number9
DOIs
Publication statusPublished - 7 Sep 2012

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Porphyromonas gingivalis
Treponema denticola
Biofilms
Proteomics
Iron
Chronic Periodontitis
Proteins
Bacterial Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Heme
Glycine
Labeling
Polyacrylamide Gel Electrophoresis
Glutamic Acid
Peptide Hydrolases
Gels
Enzymes

Keywords

  • O
  • bacterial community
  • chronic periodontitis
  • glycine
  • iron
  • LC-MALDI-TOF/TOF
  • Porphyromonas gingivalis
  • protein secretion
  • quantitative proteomics
  • Tannerella forsythia
  • Treponema denticola

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Zainal Abidin, Z., Veith, P. D., Dashper, S. G., Zhu, Y., Catmull, D. V., Chen, Y. Y., ... Reynolds, E. C. (2012). Differential proteomic analysis of a polymicrobial biofilm. Journal of Proteome Research, 11(9), 4449-4464. https://doi.org/10.1021/pr300201c

Differential proteomic analysis of a polymicrobial biofilm. / Zainal Abidin, Zamirah; Veith, Paul D.; Dashper, Stuart G.; Zhu, Ying; Catmull, Deanne V.; Chen, Yu Yen; Heryanto, Deasy C.; Chen, Dina; Pyke, James S.; Tan, Kheng; Mitchell, Helen L.; Reynolds, Eric C.

In: Journal of Proteome Research, Vol. 11, No. 9, 07.09.2012, p. 4449-4464.

Research output: Contribution to journalArticle

Zainal Abidin, Z, Veith, PD, Dashper, SG, Zhu, Y, Catmull, DV, Chen, YY, Heryanto, DC, Chen, D, Pyke, JS, Tan, K, Mitchell, HL & Reynolds, EC 2012, 'Differential proteomic analysis of a polymicrobial biofilm', Journal of Proteome Research, vol. 11, no. 9, pp. 4449-4464. https://doi.org/10.1021/pr300201c
Zainal Abidin Z, Veith PD, Dashper SG, Zhu Y, Catmull DV, Chen YY et al. Differential proteomic analysis of a polymicrobial biofilm. Journal of Proteome Research. 2012 Sep 7;11(9):4449-4464. https://doi.org/10.1021/pr300201c
Zainal Abidin, Zamirah ; Veith, Paul D. ; Dashper, Stuart G. ; Zhu, Ying ; Catmull, Deanne V. ; Chen, Yu Yen ; Heryanto, Deasy C. ; Chen, Dina ; Pyke, James S. ; Tan, Kheng ; Mitchell, Helen L. ; Reynolds, Eric C. / Differential proteomic analysis of a polymicrobial biofilm. In: Journal of Proteome Research. 2012 ; Vol. 11, No. 9. pp. 4449-4464.
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