Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts

Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani, Wan Zurinah Wan Ngah

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger accelerated cellular senescence. In this study, a SIPS model was established by exposing human diploid fibroblasts (HDFs) to 20 μM H2O2 for 2 weeks. A proteomic comparison between young, senescent and SIPS cells was done using two dimensional gel electrophoresis (2DGE) to elucidate the changes in protein expression associated with cellular aging. Our analysis showed that 28 protein spots were differentially expressed in senescent cells whereas 10 protein spots were differentially expressed in SIPS as compared to young cells. Three similar protein spots were differentially expressed in both senescent and SIPS cells when compared to the young cells. These results indicate that a difference in protein expression exists between senescent cells and SIPS cells compared to young cells.

Original languageEnglish
Pages (from-to)1247-1253
Number of pages7
JournalSains Malaysiana
Volume40
Issue number11
Publication statusPublished - Nov 2011

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Cell Aging
Fibroblasts
Skin
Proteins
Diploidy
Electrophoresis, Gel, Two-Dimensional
Oxidants
Proteomics
Growth

Keywords

  • Fibroblast
  • Senescent cells
  • SIPS
  • Two dimensional gel electrophoresis (2DGE)

ASJC Scopus subject areas

  • General

Cite this

Differential protein expression in senescent human skin fibroblasts and stress induced premature senescence (SIPS) fibroblasts. / Jo Aan, Goon; Hairi, Haryati Ahmad; Makpol, Suzana; Abdul Rahman, Mariati; Karsani, Saiful Anuar; Ngah, Wan Zurinah Wan.

In: Sains Malaysiana, Vol. 40, No. 11, 11.2011, p. 1247-1253.

Research output: Contribution to journalArticle

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AU - Karsani, Saiful Anuar

AU - Ngah, Wan Zurinah Wan

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AB - Replicative senescence of human diploid fibroblasts (HDFs) occurs when cells lose their capacity to proliferate and enter a phase of irreversible growth arrest. Stress-induced premature senescence (SIPS) on the other hand is caused by subcytotoxic concentrations of various oxidants which trigger accelerated cellular senescence. In this study, a SIPS model was established by exposing human diploid fibroblasts (HDFs) to 20 μM H2O2 for 2 weeks. A proteomic comparison between young, senescent and SIPS cells was done using two dimensional gel electrophoresis (2DGE) to elucidate the changes in protein expression associated with cellular aging. Our analysis showed that 28 protein spots were differentially expressed in senescent cells whereas 10 protein spots were differentially expressed in SIPS as compared to young cells. Three similar protein spots were differentially expressed in both senescent and SIPS cells when compared to the young cells. These results indicate that a difference in protein expression exists between senescent cells and SIPS cells compared to young cells.

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