Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds

Ismail Rozila, Pedram Azari, Sha'Ban Munirah, Wan Kamarul Zaman Wan Safwani, Seng Neon Gan, Abdul Ghani Nur Azurah, Jeevanan Jahendran, Belinda Pingguan-Murphy, Kien Hui Chua

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB-BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic-related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB-BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other co-culture groups. This result also suggests that the use of electrospun PHB-BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs.

Original languageEnglish
JournalJournal of Biomedical Materials Research - Part A
DOIs
Publication statusAccepted/In press - 2015

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Osteoblasts
Stem cells
Scaffolds
Durapatite
Hydroxyapatite
Cell culture
Assays
Phosphatases
Cell proliferation
Gene expression
Alkaline Phosphatase
Integrin-Binding Sialoprotein
Alizarin
Osteopontin
Transcription factors
3-Hydroxybutyric Acid
Osteocalcin
Cell adhesion
Scaffolds (biology)
Field emission

Keywords

  • Co-culture system
  • Electrospun
  • Human adipose-derived stem cells
  • Human osteoblasts
  • Hydroxyapatite
  • Osteogenesis
  • Polyhydroxybutyrate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds. / Rozila, Ismail; Azari, Pedram; Munirah, Sha'Ban; Wan Safwani, Wan Kamarul Zaman; Gan, Seng Neon; Nur Azurah, Abdul Ghani; Jahendran, Jeevanan; Pingguan-Murphy, Belinda; Chua, Kien Hui.

In: Journal of Biomedical Materials Research - Part A, 2015.

Research output: Contribution to journalArticle

Rozila, Ismail ; Azari, Pedram ; Munirah, Sha'Ban ; Wan Safwani, Wan Kamarul Zaman ; Gan, Seng Neon ; Nur Azurah, Abdul Ghani ; Jahendran, Jeevanan ; Pingguan-Murphy, Belinda ; Chua, Kien Hui. / Differential osteogenic potential of human adipose-derived stem cells co-cultured with human osteoblasts on polymeric microfiber scaffolds. In: Journal of Biomedical Materials Research - Part A. 2015.
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abstract = "The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB-BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic-related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB-BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other co-culture groups. This result also suggests that the use of electrospun PHB-BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs.",
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AU - Rozila, Ismail

AU - Azari, Pedram

AU - Munirah, Sha'Ban

AU - Wan Safwani, Wan Kamarul Zaman

AU - Gan, Seng Neon

AU - Nur Azurah, Abdul Ghani

AU - Jahendran, Jeevanan

AU - Pingguan-Murphy, Belinda

AU - Chua, Kien Hui

PY - 2015

Y1 - 2015

N2 - The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB-BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic-related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB-BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other co-culture groups. This result also suggests that the use of electrospun PHB-BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs.

AB - The osteogenic potential of human adipose-derived stem cells (HADSCs) co-cultured with human osteoblasts (HOBs) using selected HADSCs/HOBs ratios of 1:1, 2:1, and 1:2, respectively, is evaluated. The HADSCs/HOBs were seeded on electrospun three-dimensional poly[(R)-3-hydroxybutyric acid] (PHB) blended with bovine-derived hydroxyapatite (BHA). Monocultures of HADSCs and HOBs were used as control groups. The effects of PHB-BHA scaffold on cell proliferation and cell morphology were assessed by AlamarBlue assay and field emission scanning electron microscopy. Cell differentiation, cell mineralization, and osteogenic-related gene expression of co-culture HADSCs/HOBs were examined by alkaline phosphatase (ALP) assay, alizarin Red S assay, and quantitative real time PCR, respectively. The results showed that co-culture of HADSCs/HOBs, 1:1 grown into PHB-BHA promoted better cell adhesion, displayed a significant higher cell proliferation, higher production of ALP, extracellular mineralization and osteogenic-related gene expression of run-related transcription factor, bone sialoprotein, osteopontin, and osteocalcin compared to other co-culture groups. This result also suggests that the use of electrospun PHB-BHA in a co-culture HADSCs/HOBs system may serve as promising approach to facilitate osteogenic differentiation activity of HADSCs through direct cell-to-cell contact with HOBs.

KW - Co-culture system

KW - Electrospun

KW - Human adipose-derived stem cells

KW - Human osteoblasts

KW - Hydroxyapatite

KW - Osteogenesis

KW - Polyhydroxybutyrate

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