Differential osteogenic activity of osteoprogenitor cells on HA and TCP/HA scaffold of tissue engineered bone

Min Hwei Ng, K. K. Tan, M. Y. Phang, O. Aziyati, G. H. Tan, M. R. Isa, B. S. Aminuddin, M. Naseem, O. Fauziah, Ruszymah Idrus

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Biomaterial, an essential component of tissue engineering, serves as a scaffold for cell attachment, proliferation, and differentiation; provides the three dimensional (3D) structure and, in some applications, the mechanical strength required for the engineered tissue. Both synthetic and naturally occurring calcium phosphate based biomaterial have been used as bone fillers or bone extenders in orthopedic and reconstructive surgeries. This study aims to evaluate two popular calcium phosphate based biomaterial i.e., hydroxyapatite (HA) and tricalcium phosphate/hydroxyapatite (TCP/HA) granules as scaffold materials in bone tissue engineering. In our strategy for constructing tissue engineered bone, human osteoprogenitor cells derived from periosteum were incorporated with human plasma-derived fibrin and seeded onto HA or TCP/HA forming 3D tissue constructs and further maintained in osteogenic medium for 4 weeks to induce osteogenic differentiation. Constructs were subsequently implanted intramuscularly in nude mice for 8 weeks after which mice were euthanized and constructs harvested for evaluation. The differential cell response to the biomaterial (HA or TCP/HA) adopted as scaffold was illustrated by the histology of undecalcified constructs and evaluation using SEM and TEM. Both HA and TCP/HA constructs showed evidence of cell proliferation, calcium deposition, and collagen bundle formation albeit lesser in the former. Our findings demonstrated that TCP/HA is superior between the two in early bone formation and hence is the scaffold material of choice in bone tissue engineering.

Original languageEnglish
Pages (from-to)301-312
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume85
Issue number2
DOIs
Publication statusPublished - May 2008

Fingerprint

Tissue Scaffolds
Durapatite
Hydroxyapatite
Scaffolds
Bone
Phosphates
Tissue
Biocompatible Materials
Scaffolds (biology)
Biomaterials
Tissue engineering
Calcium phosphate
Plasma (human)
tricalcium phosphate
Histology
Orthopedics
Cell proliferation
Fibrin
Collagen
Surgery

Keywords

  • Bone tissue engineering
  • Fibrin
  • Hydroxyapatite
  • Periosteum
  • Tricalcium phosphate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Differential osteogenic activity of osteoprogenitor cells on HA and TCP/HA scaffold of tissue engineered bone. / Ng, Min Hwei; Tan, K. K.; Phang, M. Y.; Aziyati, O.; Tan, G. H.; Isa, M. R.; Aminuddin, B. S.; Naseem, M.; Fauziah, O.; Idrus, Ruszymah.

In: Journal of Biomedical Materials Research - Part A, Vol. 85, No. 2, 05.2008, p. 301-312.

Research output: Contribution to journalArticle

Ng, Min Hwei ; Tan, K. K. ; Phang, M. Y. ; Aziyati, O. ; Tan, G. H. ; Isa, M. R. ; Aminuddin, B. S. ; Naseem, M. ; Fauziah, O. ; Idrus, Ruszymah. / Differential osteogenic activity of osteoprogenitor cells on HA and TCP/HA scaffold of tissue engineered bone. In: Journal of Biomedical Materials Research - Part A. 2008 ; Vol. 85, No. 2. pp. 301-312.
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