Differential effect of adrenocorticosteroids on 11β-hydroxysteroid dehydrogenase bioactivity at the anterior pituitary and hypothalamus in rats

Ruszymah Idrus, Nabishah Mohamad, Paden B. Morat, Aminuddin Saim, Khalid B. Abdul Kadir

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

11β-Hydroxysteroid dehydrogenase (11β-OHSD) is an microsomal enzyme that catalyzes the dehydrogenation of cortisol (F) to cortisone(E) in man and corticosterone (B) to 11-dehydrocorticosterone (A) in rats. 11β-OHSD has been identified in a wide variety of tissues. The differential distribution of 11β-OHSD suggests that this enzyme has locally defined functions that vary from region to region. The aim of this study was to investigate the effects of the glucocorticoids B and dexamethasone (DM), the mineralocorticoid deoxycorticosterone (DOC), and the inhibitors of 11β-OHSD glycyrrhizic acid (GI) and glycyrrhetinic acid (GE) on 111β-OHSD bioactivity at the hypothalamus (HT) and anterior pituitary (AP). Male Wistar rats were treated with GI or were adrenalectomized (ADX) and treated with either B, DM, or DOC for 7 days. All treatments were in vivo except GE, which was used in vitro. At the end of treatment, homogenates of HT and AP were assayed for 11β-OHSD bioactivity, expressed as the percentage conversion of B to A in the presence of NADP. 11β-OHSD bioactivity is significantly higher (P < 0.0001) in the AP compared with the HT. Adrenalectomy significantly increased the enzyme activity in the AP (P < 0.05), an effect reversed by B or DM. ADX rats treated with DOC showed decreased enzyme activity in the AP (P < 0.001) but increased the activity in the HT (P < 0.0001). GI increased activity in both HT and AP, whereas GE decreased activity significantly. We conclude that the modulation of 11β-OHSD is both steroid specific and tissue specific.

Original languageEnglish
Pages (from-to)448-452
Number of pages5
JournalSteroids
Volume61
Issue number8
DOIs
Publication statusPublished - Aug 1996

Fingerprint

Glycyrrhetinic Acid
11-beta-Hydroxysteroid Dehydrogenases
Glycyrrhizic Acid
Anterior Hypothalamus
Desoxycorticosterone
Bioactivity
Dexamethasone
Rats
Enzyme activity
Enzymes
Tissue
Hypothalamus
Mineralocorticoids
Cortisone
Dehydrogenation
Corticosterone
NADP
Glucocorticoids
Hydrocortisone
Adrenalectomy

Keywords

  • 11β-OHSD bioactivity
  • anterior pituitary
  • glycyrrhizic acid
  • hypothalamus
  • rat

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Differential effect of adrenocorticosteroids on 11β-hydroxysteroid dehydrogenase bioactivity at the anterior pituitary and hypothalamus in rats. / Idrus, Ruszymah; Mohamad, Nabishah; Morat, Paden B.; Saim, Aminuddin; Abdul Kadir, Khalid B.

In: Steroids, Vol. 61, No. 8, 08.1996, p. 448-452.

Research output: Contribution to journalArticle

Idrus, Ruszymah ; Mohamad, Nabishah ; Morat, Paden B. ; Saim, Aminuddin ; Abdul Kadir, Khalid B. / Differential effect of adrenocorticosteroids on 11β-hydroxysteroid dehydrogenase bioactivity at the anterior pituitary and hypothalamus in rats. In: Steroids. 1996 ; Vol. 61, No. 8. pp. 448-452.
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