Differences in protein changes between stress-induced premature senescence and replicative senescence states

Goon Jo Aan, Haryati Ahmad Hairi, Suzana Makpol, Mariati Abdul Rahman, Saiful Anuar Karsani

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Replicative senescence and stress-induced premature senescence (SIPS) cells are known to share certain traits. However, whether these cells are different at the protein level is unclear. Thus, this study has utilized proteomics to identify differences in the proteomes of replicative senescence and SIPS cells compared to normal cells. Replicative senescence was induced by serial passage of normal cells in culture. SIPS was established by exposure to H2O2 at a subcytotoxic concentration of 20 μM for two weeks. Following 2DE, protein profiles were compared and protein spots that changed in abundance were identified by MALDI-TOF MS. Quantitative real-time RT-PCR was then performed to evaluate the transcript expression of selected altered proteins. A total of 24 and 10 proteins were found to have changed in abundance in replicative senescence and SIPS cells, respectively, when compared to young cells. Quantitative RT-PCR revealed that nine genes showed the same direction of change as observed in the proteomics analysis. Very little overlap was observed between proteins that changed in replicative senescence and SIPS cells, suggesting that although both SIPS and replicative senescence cells share hallmarks of cellular senescence, they were different in terms of proteins that changed in abundance.

Original languageEnglish
Pages (from-to)2209-2217
Number of pages9
JournalElectrophoresis
Volume34
Issue number15
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Cell Aging
Proteins
Proteomics
Proteome
Serial Passage
Genes
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Real-Time Polymerase Chain Reaction
Cell Culture Techniques
Polymerase Chain Reaction

Keywords

  • Fibroblasts
  • MALDI-TOF MS/MS
  • Proteomics
  • Senescence

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Differences in protein changes between stress-induced premature senescence and replicative senescence states. / Jo Aan, Goon; Hairi, Haryati Ahmad; Makpol, Suzana; Abdul Rahman, Mariati; Karsani, Saiful Anuar.

In: Electrophoresis, Vol. 34, No. 15, 08.2013, p. 2209-2217.

Research output: Contribution to journalArticle

@article{24e674fce2a34a2da109386f3e9da97d,
title = "Differences in protein changes between stress-induced premature senescence and replicative senescence states",
abstract = "Replicative senescence and stress-induced premature senescence (SIPS) cells are known to share certain traits. However, whether these cells are different at the protein level is unclear. Thus, this study has utilized proteomics to identify differences in the proteomes of replicative senescence and SIPS cells compared to normal cells. Replicative senescence was induced by serial passage of normal cells in culture. SIPS was established by exposure to H2O2 at a subcytotoxic concentration of 20 μM for two weeks. Following 2DE, protein profiles were compared and protein spots that changed in abundance were identified by MALDI-TOF MS. Quantitative real-time RT-PCR was then performed to evaluate the transcript expression of selected altered proteins. A total of 24 and 10 proteins were found to have changed in abundance in replicative senescence and SIPS cells, respectively, when compared to young cells. Quantitative RT-PCR revealed that nine genes showed the same direction of change as observed in the proteomics analysis. Very little overlap was observed between proteins that changed in replicative senescence and SIPS cells, suggesting that although both SIPS and replicative senescence cells share hallmarks of cellular senescence, they were different in terms of proteins that changed in abundance.",
keywords = "Fibroblasts, MALDI-TOF MS/MS, Proteomics, Senescence",
author = "{Jo Aan}, Goon and Hairi, {Haryati Ahmad} and Suzana Makpol and {Abdul Rahman}, Mariati and Karsani, {Saiful Anuar}",
year = "2013",
month = "8",
doi = "10.1002/elps.201300086",
language = "English",
volume = "34",
pages = "2209--2217",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "15",

}

TY - JOUR

T1 - Differences in protein changes between stress-induced premature senescence and replicative senescence states

AU - Jo Aan, Goon

AU - Hairi, Haryati Ahmad

AU - Makpol, Suzana

AU - Abdul Rahman, Mariati

AU - Karsani, Saiful Anuar

PY - 2013/8

Y1 - 2013/8

N2 - Replicative senescence and stress-induced premature senescence (SIPS) cells are known to share certain traits. However, whether these cells are different at the protein level is unclear. Thus, this study has utilized proteomics to identify differences in the proteomes of replicative senescence and SIPS cells compared to normal cells. Replicative senescence was induced by serial passage of normal cells in culture. SIPS was established by exposure to H2O2 at a subcytotoxic concentration of 20 μM for two weeks. Following 2DE, protein profiles were compared and protein spots that changed in abundance were identified by MALDI-TOF MS. Quantitative real-time RT-PCR was then performed to evaluate the transcript expression of selected altered proteins. A total of 24 and 10 proteins were found to have changed in abundance in replicative senescence and SIPS cells, respectively, when compared to young cells. Quantitative RT-PCR revealed that nine genes showed the same direction of change as observed in the proteomics analysis. Very little overlap was observed between proteins that changed in replicative senescence and SIPS cells, suggesting that although both SIPS and replicative senescence cells share hallmarks of cellular senescence, they were different in terms of proteins that changed in abundance.

AB - Replicative senescence and stress-induced premature senescence (SIPS) cells are known to share certain traits. However, whether these cells are different at the protein level is unclear. Thus, this study has utilized proteomics to identify differences in the proteomes of replicative senescence and SIPS cells compared to normal cells. Replicative senescence was induced by serial passage of normal cells in culture. SIPS was established by exposure to H2O2 at a subcytotoxic concentration of 20 μM for two weeks. Following 2DE, protein profiles were compared and protein spots that changed in abundance were identified by MALDI-TOF MS. Quantitative real-time RT-PCR was then performed to evaluate the transcript expression of selected altered proteins. A total of 24 and 10 proteins were found to have changed in abundance in replicative senescence and SIPS cells, respectively, when compared to young cells. Quantitative RT-PCR revealed that nine genes showed the same direction of change as observed in the proteomics analysis. Very little overlap was observed between proteins that changed in replicative senescence and SIPS cells, suggesting that although both SIPS and replicative senescence cells share hallmarks of cellular senescence, they were different in terms of proteins that changed in abundance.

KW - Fibroblasts

KW - MALDI-TOF MS/MS

KW - Proteomics

KW - Senescence

UR - http://www.scopus.com/inward/record.url?scp=84881249955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881249955&partnerID=8YFLogxK

U2 - 10.1002/elps.201300086

DO - 10.1002/elps.201300086

M3 - Article

VL - 34

SP - 2209

EP - 2217

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 15

ER -