Dielectrophoretic deformation of breast cancer cells for lab on a chip applications

Jun Yuan Chan, Aminuddin Bin Ahmad Kayani, Mohd Anuar Md Ali, Chee Kuang Kok, Muhamad Ramdzan Buyong, Susan Ling Ling Hoe, Marini Marzuki, Alan Soo-Beng Khoo, Sharath Sriram, Kostya Ostrikov

Research output: Contribution to journalArticle

Abstract

This paper presents the development and experimental analysis of a curved microelectrode platform for the DEP deformation of breast cancer cells (MDA-MB-231). The platform is composed of arrays of curved DEP microelectrodes which are patterned onto a glass slide and samples containing MDA-MB-231 cells are pipetted onto the platform's surface. Finite element method is utilised to characterise the electric field gradient and DEP field. The performance of the system is assessed with MDA-MB-231 cells in a low conductivity 1% DMEM suspending medium. We applied sinusoidal wave AC potential at peak to peak voltages of 2, 5, and 10 Vpp at both 10 kHz and 50 MHz. We observed cell blebbing and cell shrinkage and analyzed the percentage of shrinkage of the cells. The experiments demonstrated higher percentage of cell shrinkage when cells are exposed to higher frequency and peak to peak voltage electric field.

Original languageEnglish
Pages (from-to)2728-2735
Number of pages8
JournalElectrophoresis
Volume40
Issue number20
DOIs
Publication statusPublished - 1 Oct 2019

Fingerprint

Lab-on-a-chip
Microelectrodes
Cells
Breast Neoplasms
Electric fields
Electric potential
Finite element method
Glass
1-(2-(dodecyloxy)ethyl)pyrrolidine hydrochloride
Blister
Experiments

Keywords

  • Blebbing
  • Cancer
  • Cell shrinkage
  • Deformation
  • Dielectrophoresis
  • Microfluidics

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Chan, J. Y., Ahmad Kayani, A. B., Md Ali, M. A., Kok, C. K., Ramdzan Buyong, M., Hoe, S. L. L., ... Ostrikov, K. (2019). Dielectrophoretic deformation of breast cancer cells for lab on a chip applications. Electrophoresis, 40(20), 2728-2735. https://doi.org/10.1002/elps.201800442

Dielectrophoretic deformation of breast cancer cells for lab on a chip applications. / Chan, Jun Yuan; Ahmad Kayani, Aminuddin Bin; Md Ali, Mohd Anuar; Kok, Chee Kuang; Ramdzan Buyong, Muhamad; Hoe, Susan Ling Ling; Marzuki, Marini; Soo-Beng Khoo, Alan; Sriram, Sharath; Ostrikov, Kostya.

In: Electrophoresis, Vol. 40, No. 20, 01.10.2019, p. 2728-2735.

Research output: Contribution to journalArticle

Chan, JY, Ahmad Kayani, AB, Md Ali, MA, Kok, CK, Ramdzan Buyong, M, Hoe, SLL, Marzuki, M, Soo-Beng Khoo, A, Sriram, S & Ostrikov, K 2019, 'Dielectrophoretic deformation of breast cancer cells for lab on a chip applications', Electrophoresis, vol. 40, no. 20, pp. 2728-2735. https://doi.org/10.1002/elps.201800442
Chan, Jun Yuan ; Ahmad Kayani, Aminuddin Bin ; Md Ali, Mohd Anuar ; Kok, Chee Kuang ; Ramdzan Buyong, Muhamad ; Hoe, Susan Ling Ling ; Marzuki, Marini ; Soo-Beng Khoo, Alan ; Sriram, Sharath ; Ostrikov, Kostya. / Dielectrophoretic deformation of breast cancer cells for lab on a chip applications. In: Electrophoresis. 2019 ; Vol. 40, No. 20. pp. 2728-2735.
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