Dielectrophoresis of amyloid-beta proteins as a microfluidic template for alzheimer’s research

Salman Ali Al-Ahdal, Aminuddin Bin Ahmad Kayani, Mohd Anuar Md Ali, Jun Yuan Chan, Talal Ali, Norah Adnan, Muhamad Ramdzan Buyong, Ervina Efzan Mhd Noor, Burhanuddin Yeop Majlis, Sharath Sriram

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We employed dielectrophoresis to a yeast cell suspension containing amyloid-beta proteins (Aβ) in a microfluidic environment. The Aβ was separated from the cells and characterized using the gradual dissolution of Aβ as a function of the applied dielectrophoretic parameters. We established the gradual dissolution of Aβ under specific dielectrophoretic parameters. Further, Aβ in the fibril form at the tip of the electrode dissolved at high frequency. This was perhaps due to the conductivity of the suspending medium changing according to the frequency, which resulted in a higher temperature at the tips of the electrodes, and consequently in the breakdown of the hydrogen bonds. However, those shaped as spheroidal monomers experienced a delay in the Aβ fibril transformation process. Yeast cells exposed to relatively low temperatures at the base of the electrode did not experience a positive or negative change in viability. The DEP microfluidic platform incorporating the integrated microtip electrode array was able to selectively manipulate the yeast cells and dissolve the Aβ to a controlled extent. We demonstrate suitable dielectrophoretic parameters to induce such manipulation, which is highly relevant for Aβ-related colloidal microfluidic research and could be applied to Alzheimer’s research in the future.

Original languageEnglish
Article number3595
JournalInternational journal of molecular sciences
Volume20
Issue number14
DOIs
Publication statusPublished - 2 Jul 2019

Fingerprint

Microfluidics
Amyloid beta-Peptides
Electrophoresis
yeast
Electrodes
templates
Yeast
proteins
Proteins
electrodes
Yeasts
Cells
Research
dissolving
Dissolution
Temperature
Staphylococcal Protein A
viability
manipulators
Hydrogen

Keywords

  • Bioelectric
  • Dielectrophoresis
  • Lab-on-a-chip
  • Microanalysis
  • Microfabrication
  • Microfluidics

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Al-Ahdal, S. A., Kayani, A. B. A., Ali, M. A. M., Chan, J. Y., Ali, T., Adnan, N., ... Sriram, S. (2019). Dielectrophoresis of amyloid-beta proteins as a microfluidic template for alzheimer’s research. International journal of molecular sciences, 20(14), [3595]. https://doi.org/10.3390/ijms20143595

Dielectrophoresis of amyloid-beta proteins as a microfluidic template for alzheimer’s research. / Al-Ahdal, Salman Ali; Kayani, Aminuddin Bin Ahmad; Ali, Mohd Anuar Md; Chan, Jun Yuan; Ali, Talal; Adnan, Norah; Buyong, Muhamad Ramdzan; Noor, Ervina Efzan Mhd; Yeop Majlis, Burhanuddin; Sriram, Sharath.

In: International journal of molecular sciences, Vol. 20, No. 14, 3595, 02.07.2019.

Research output: Contribution to journalArticle

Al-Ahdal, Salman Ali ; Kayani, Aminuddin Bin Ahmad ; Ali, Mohd Anuar Md ; Chan, Jun Yuan ; Ali, Talal ; Adnan, Norah ; Buyong, Muhamad Ramdzan ; Noor, Ervina Efzan Mhd ; Yeop Majlis, Burhanuddin ; Sriram, Sharath. / Dielectrophoresis of amyloid-beta proteins as a microfluidic template for alzheimer’s research. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 14.
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