Developmental abnormalities and reduced fruit softening in tomato plants expressing an antisense rab11 GTPase gene

C. Lu, Zamri Zainal, G. A. Tucker, G. W. Lycett

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

A cDNA clone from tomato fruit encodes a protein with strong homology with the rab11/YPT3 class of small GTPases that is thought to be involved in the control of protein trafficking within cells. The gene, LeRab11a, showed a pattern consistent with a single copy in DNA gel blots. The corresponding mRNA was developmentally regulated during fruit ripening, and its expression was inhibited in several ripening mutants. Its reduced expression in the Never-ripe mutant indicates that it may be induced by ethylene in fruit. The ripening-induced expression in tissues that are undergoing cell wall loosening immediately suggests a possible role in trafficking of cell wall-modifying enzymes. The message also was produced in leaves and flowers but not in roots. Antisense transformation was used to generate a "mutant phenotype." Antisense fruit changed color as expected but failed to soften normally. This was accompanied by reduced levels of two cell wall hydrolases, pectinesterase and polygalacturonase. There were other phenotypic effects in the plants, including determinate growth, reduced apical dominance, branched inflorescences, abnormal floral structure, and ectopic shoots on the leaves. In some plants, ethylene production was reduced. These data suggest an alternative or additional role in exocytosis or endocytosis of homeotic proteins, hormone carriers, or receptors.

Original languageEnglish
Pages (from-to)1819-1833
Number of pages15
JournalPlant Cell
Volume13
Issue number8
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

GTP Phosphohydrolases
abnormal development
guanosinetriphosphatase
Lycopersicon esculentum
Fruits
Fruit
Genes
tomatoes
Cell Wall
ripening
fruits
Cells
cell walls
mutants
genes
homeodomain proteins
determinate growth
Homeodomain Proteins
Polygalacturonase
Inflorescence

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Developmental abnormalities and reduced fruit softening in tomato plants expressing an antisense rab11 GTPase gene. / Lu, C.; Zainal, Zamri; Tucker, G. A.; Lycett, G. W.

In: Plant Cell, Vol. 13, No. 8, 2001, p. 1819-1833.

Research output: Contribution to journalArticle

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