Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds

Effect on morphology, mechanical strength, biostability and cytocompatibility

Saleem Ullah, Ismail Zainol, Shiplu Roy Chowdhury, M. B. Fauzi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility. The scaffolds were fabricated via freeze-drying technique. The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically. The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application. The average pore sizes of scaffolds were in range of 100.73 ± 27.62–116.01 ± 52.06, porosity 71.72 ± 3.46–91.17 ± 2.42%, tensile modulus in dry environment 1.47 ± 0.08–0.17 ± 0.03 MPa, tensile modulus in wet environment 0.32 ± 0.03–0.14 ± 0.04 MPa and biodegradation rate (at day 30) 60.38 ± 0.70–83.48 ± 0.28%. In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion. It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.

Original languageEnglish
Pages (from-to)158-168
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume111
DOIs
Publication statusPublished - 1 May 2018

Fingerprint

Chitosan
Scaffolds (biology)
Scaffolds
Glycerol
Fish
Strength of materials
Porosity
Fishes
Collagen
Chemical analysis
Elastic moduli
Freeze Drying
Tissue Engineering
Keratinocytes
Cell Adhesion
Cell adhesion
Cell proliferation
Fibroblasts
Biodegradation
Tissue engineering

Keywords

  • 3D porous scaffolds
  • Cytocompatibility
  • Mechanical properties
  • Microstructure
  • Multicomponent scaffolds
  • Tissue engineering

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Cite this

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title = "Development of various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds: Effect on morphology, mechanical strength, biostability and cytocompatibility",
abstract = "The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility. The scaffolds were fabricated via freeze-drying technique. The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically. The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application. The average pore sizes of scaffolds were in range of 100.73 ± 27.62–116.01 ± 52.06, porosity 71.72 ± 3.46–91.17 ± 2.42{\%}, tensile modulus in dry environment 1.47 ± 0.08–0.17 ± 0.03 MPa, tensile modulus in wet environment 0.32 ± 0.03–0.14 ± 0.04 MPa and biodegradation rate (at day 30) 60.38 ± 0.70–83.48 ± 0.28{\%}. In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion. It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.",
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AU - Chowdhury, Shiplu Roy

AU - Fauzi, M. B.

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AB - The various composition multicomponent chitosan/fish collagen/glycerin 3D porous scaffolds were developed and investigated the effect of various composition chitosan/fish collagen/glycerin on scaffolds morphology, mechanical strength, biostability and cytocompatibility. The scaffolds were fabricated via freeze-drying technique. The effects of various compositions consisting in 3D scaffolds were investigated via FT-IR analysis, porosity, swelling and mechanical tests, and effect on the morphology of scaffolds investigated microscopically. The biostability and cytocompatibility tests were used to explore the ability of scaffolds to use for tissue engineering application. The average pore sizes of scaffolds were in range of 100.73 ± 27.62–116.01 ± 52.06, porosity 71.72 ± 3.46–91.17 ± 2.42%, tensile modulus in dry environment 1.47 ± 0.08–0.17 ± 0.03 MPa, tensile modulus in wet environment 0.32 ± 0.03–0.14 ± 0.04 MPa and biodegradation rate (at day 30) 60.38 ± 0.70–83.48 ± 0.28%. In vitro culture of human fibroblasts and keratinocytes showed that the various composition multicomponent 3D scaffolds were good cytocompatibility however, the scaffolds contained high amount of fish collagen excellently facilitated cell proliferation and adhesion. It was found that the high amount fish collagen and glycerin scaffolds have high porosity, enough mechanical strength and biostability, and excellent cytocompatibility.

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