Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability

Mohd Iswadi Ismail, Khairul Osman, Siti Fatimah Ibrahim, Jaffar Farah Hanan Fathihah, Nur Azianie Abd Ghani, Fazly Ann Zainalabidin, Abas Mazni Othman

Research output: Contribution to journalArticle

Abstract

The objective of this study was to evaluate the potential of using single tubing-type canister on sperm quality. Semen was collected from the Bali cattle bull by electroejaculation technique and was cryopreserved in liquid nitrogen using slow freezing cryopreservation method. Two type of canister volume was used in this study; commercial canister (342.25p x 278 mm2) and single tubing-type canister (4p x 90 mm2). Makler counting chamber and computer assisted sperm analyzer (CASA) were used to evaluate the sperm motility and viability of post-thaw sperm. Results showed that the bull sperm motility and viability at the bottom of tubing-type canister was statistically higher and significant as compared to the commercial canister (p<0.05). Significant changes were found in sperm kinetics (VCL, VAP, VSL) of tubing-type canister compared to commercial canister. No significant changes in the motility and viability of the bull sperm at the top of tubing-type canister and commercial canister. There were no significant changes in sperm progression (LIN, WOB, PROG) in both the canisters. Developed tubing-type canister in this study showed potential as an alternative to be used in bull sperm cryo-storage.

Original languageEnglish
Pages (from-to)194-199
Number of pages6
JournalBrazilian Archives of Biology and Technology
Volume57
Issue number2
DOIs
Publication statusPublished - 2014

Fingerprint

sperm motility
semen
bulls
spermatozoa
viability
cryopreservation
Indonesia
freezing
kinetics
liquids
cattle
nitrogen
methodology

Keywords

  • Canister
  • Cryopreservation
  • Motility
  • Sperm
  • Viability

ASJC Scopus subject areas

  • General

Cite this

Development of single tubing-type canister for cryo-storage of bull semen and their effect on sperm motility and viability. / Ismail, Mohd Iswadi; Osman, Khairul; Ibrahim, Siti Fatimah; Farah Hanan Fathihah, Jaffar; Ghani, Nur Azianie Abd; Zainalabidin, Fazly Ann; Othman, Abas Mazni.

In: Brazilian Archives of Biology and Technology, Vol. 57, No. 2, 2014, p. 194-199.

Research output: Contribution to journalArticle

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