Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate

Lee Lih Fen, Rosli Md.Illias, Kamarulzaman Kamaruddin, Mohamad Yusof Maskat, Osman Hassan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chitosanolytic activity was monitored using chitosan impregnated with Remazol Brilliant Blue R dye (RBB). Low-yielding chitosan (1%, w/v) hydrolysis was modelled using Viscozyme® L (50%, w/w), a commercial mixed-glucanase, and was found to perform optimally at pH 4.5 and 50°C. The chitosanase assay was performed on powdered (RBB-P) and colloidal form (RBB-C) of the dyed chitosan. Hydrolysis of chitosan was accompanied by the release of water-soluble dyed-hydrolysate, which can be monitored rapidly using microplate reader at 595 nm. The result obtained from this method was in agreement with the determination of reducing end by using the traditional, complex and time-consuming DNS method. The method is significantly rapid and more sensitive than DNS method.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
JournalEnzyme and Microbial Technology
Volume38
Issue number1-2
DOIs
Publication statusPublished - 3 Jan 2006

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chitosanase
Chitosan
Screening
Substrates
Hydrolysis
Assays
Coloring Agents
Dyes
brilliant blue
Water

Keywords

  • Chitosanase
  • Rapid method
  • RBB-chitosan

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate. / Fen, Lee Lih; Md.Illias, Rosli; Kamaruddin, Kamarulzaman; Maskat, Mohamad Yusof; Hassan, Osman.

In: Enzyme and Microbial Technology, Vol. 38, No. 1-2, 03.01.2006, p. 215-219.

Research output: Contribution to journalArticle

Fen, Lee Lih ; Md.Illias, Rosli ; Kamaruddin, Kamarulzaman ; Maskat, Mohamad Yusof ; Hassan, Osman. / Development of rapid screening method for low-yielding chitosanase activity using Remazol Brilliant Blue-chitosan as substrate. In: Enzyme and Microbial Technology. 2006 ; Vol. 38, No. 1-2. pp. 215-219.
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