Development of extraction system by using thermo-responsive polymer for Bovine Serum Albumin recovery

F. A. Abdul Mutalib, Jamaliah Md Jahim

Research output: Contribution to journalArticle

Abstract

Thermo-responsive polymers, Dehypon®LS54 and Berol®370 were paired randomly with starch derivatives, K4484®dextrin and N-Lite®D maltodextrin for develop thermoseparating ATPS. The developed systems are DehyponLS54/K4484, DehyponLS54/N-Lite.D and Berol370/K4484. All systems were tested with partitioning of BSA as model protein. Among the tested systems, DehyponLS54/K4484 was found as most suitable system for BSA recovery. A small electrochemical potential effect was observed in DehyponLS54/K4484 dextrin system in which the change of pH (pH 8 to 6) had slightly influence the partitioning behavior of BSA. At both pH, BSA was observed had high affinity to starch enriched phase and it caused a low yield value of BSA after thermoseparation step. However, recovery of BSA in water phase at pH 6 was higher 40% compared to pH 8. The addition of Na2SO4 has lowered the cloud point of Dehypon®LS54.

Original languageEnglish
Pages (from-to)3188-3191
Number of pages4
JournalJournal of Applied Sciences
Volume9
Issue number17
DOIs
Publication statusPublished - 2009

Fingerprint

bovine serum albumin
polymers
starch
dextrins
maltodextrins
chemical derivatives
proteins
water

Keywords

  • AIPS
  • Protein extraction
  • Thermo-responsive polymers

ASJC Scopus subject areas

  • General

Cite this

Development of extraction system by using thermo-responsive polymer for Bovine Serum Albumin recovery. / Abdul Mutalib, F. A.; Md Jahim, Jamaliah.

In: Journal of Applied Sciences, Vol. 9, No. 17, 2009, p. 3188-3191.

Research output: Contribution to journalArticle

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abstract = "Thermo-responsive polymers, Dehypon{\circledR}LS54 and Berol{\circledR}370 were paired randomly with starch derivatives, K4484{\circledR}dextrin and N-Lite{\circledR}D maltodextrin for develop thermoseparating ATPS. The developed systems are DehyponLS54/K4484, DehyponLS54/N-Lite.D and Berol370/K4484. All systems were tested with partitioning of BSA as model protein. Among the tested systems, DehyponLS54/K4484 was found as most suitable system for BSA recovery. A small electrochemical potential effect was observed in DehyponLS54/K4484 dextrin system in which the change of pH (pH 8 to 6) had slightly influence the partitioning behavior of BSA. At both pH, BSA was observed had high affinity to starch enriched phase and it caused a low yield value of BSA after thermoseparation step. However, recovery of BSA in water phase at pH 6 was higher 40{\%} compared to pH 8. The addition of Na2SO4 has lowered the cloud point of Dehypon{\circledR}LS54.",
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